Prof. Dr. Karin Busch
Membrane protein dynamics in bioenergetics
Cell Biology / Molecular Biology
Imaging Technology
Bioenergetics
Membrane Protein Dynamics
Our group is fascinated by the problem how mitochondria respond to different energy expenditure and demand. We think that re-shaping of cristae controlled by diverse protein complexes plays an essential role in providing plasticity and allowing for adaptive mitochondrial function. For example, respiratory complexes of mitochondria assembly into supercomplexes (Rieger et al., 2017) in response to specific (energetic) states. ATP synthase forms dimers that shape cristae. Other membrane proteins interact with soluble proteins to control the form of cristae. These interactions often are spatially and temporally restricted and thus difficult to survey. To overcome this limit and to dissect the dynamic nano-organization of mitochondrial membranes, we implemented a specific superresolution microscopy technique (TALM: tracking and localization microscopy). We investigate the role of membrane protein complexes, cristae dynamics and functional adaptation with advanced biophysical and live cell imaging methods including FRET/FLIM, apply multi-color single molecule tracking and localization microscopy and use different fluorescent biosensors for functional readouts.
Vita
- 1986-1989: Studies in Biochemistry, University Tübingen
- 1989-1993: Studies in Biology, Universität Tübingen
- 1994-1996: PhD, Universität Tübingen
- 1997-1999: Postdoctorat Weizmann Institut, Rehovot, Israel
- 1999-2000: Postdoctorat MPI Biochemie, Martinsried, München
- 2000-2003: Research Assistent Institut für Biochemie, Universities Marburg und Frankfurt
- 2004-2007: Independent Researcher, Institute for Cellbiology, University Frankfurt
- 2008-2009: Group leader, Institute for Biology, University of Osnabrück
- 2009-2015: Junior-Professor, University of Osnabrück
- since 2015: Professor Molecular Cell Biology, University of Münster
Selected references
Liebmann M, Hucke S, Koch K, Eschborn M, Ghelman J, Chasan AI, Glander S, Schadlich M, Kuhlencord M, Daber NM, Eveslage M, Beyer M, Dietrich M, Albrecht P, Stoll M, Busch KB, Wiendl H, Roth J, Kuhlmann T and Klotz L (2018). Nur77 serves as a molecular brake of the metabolic switch during T cell activation to restrict autoimmunity. Proc Natl Acad Sci U S A 115: E8017-E8026
Zalyevskiy K, Rieger B, Hager F, Richter CP, Psathaki K, Appelhans T and Busch B (2018). F1FO ATP synthase responds to glycolysis inhibition by localization into the inner boundary membrane. bioRxiv 374967; doi: https://doi.org/10.1101/374967
Appelhans T, Beinlich FRM, Richter CP, Kurre R and Busch KB (2018). Multi-color localization microscopy of single membrane proteins in organelles of live mammalian cells. J Vis Exp. 136
Rieger B, Shalaeva DN, Söhnel A-CC, Kohl W, Duwe P, Mulkidjanian AY & Busch KB (2017). Lifetime imaging of GFP at CoxVIIIa reports respiratory supercomplex assembly in live cells. Sci Rep 7, 46055.
Söhnel AC, Kohl W, Gregor I, Enderlein J, Rieger B, Busch KB (2016). Probing of protein localization and shuttling in mitochondrial microcompartments by FLIM with sub-diffraction resolution, BBA Bioenergetics 1857:1290-1299.
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