Laser | Channel | Optical Filter Setting | Fluorochrome / fluorescent Protein |
Violet | 407 A | LP 600, BP 610/20 | Qdot 605 |
405 nm | 407 B | LP 502, BP 510/50 | AmCyan |
407 C | BP 450/40 | DAPI, Hoechst 33342/33258 | |
Blue | 488 A | LP 655, BP 695/40 | PerCP-Cy5.5, PerCP |
488 nm | 488 B | LP 502, BP 530/30 | FITC, GFP, Alexa Fluor 488 |
488 C | BP 488/10 | SSC Detector | |
Yellow-Green | 561 A | LP 735, BP 780/60 | PE-Cy7 |
561 nm | 561 B | LP 685, BP 710/50 | PE Alexa 700 |
561 C | LP 630, BP 670/14 | PE-Cy5 | |
561 D | LP 600, BP 610/20 | PE-Texas Red, PI, mCherry | |
561 E | BP 582/15 | PE, tdTomato | |
Red | 633 A | LP 755, BP 780/60 | APC-Cy7 |
633 nm | 633 B | LP 690, BP 730/45 | Alexa Fluor 700 |
633 C | BP 660/20 | APC, DyLight 633 / 64 |