Laser |
Channel |
Optical Filter Setting |
Fluorochrome / fluorescent Protein |
Violet |
407 A |
LP 600, BP 610/20 |
Qdot 605 |
405 nm |
407 B |
LP 502, BP 510/50 |
AmCyan |
|
407 C |
BP 450/40 |
DAPI, Hoechst 33342/33258 |
Blue |
488 A |
LP 655, BP 695/40 |
PerCP-Cy5.5, PerCP |
488 nm |
488 B |
LP 502, BP 530/30 |
FITC, GFP, Alexa Fluor 488 |
|
488 C |
BP 488/10 |
SSC Detector |
Yellow-Green |
561 A |
LP 735, BP 780/60 |
PE-Cy7 |
561 nm |
561 B |
LP 685, BP 710/50 |
PE Alexa 700 |
|
561 C |
LP 630, BP 670/14 |
PE-Cy5 |
|
561 D |
LP 600, BP 610/20 |
PE-Texas Red, PI, mCherry |
|
561 E |
BP 582/15 |
PE, tdTomato |
Red |
633 A |
LP 755, BP 780/60 |
APC-Cy7 |
633 nm |
633 B |
LP 690, BP 730/45 |
Alexa Fluor 700 |
|
633 C |
BP 660/20 |
APC, DyLight 633 / 64 |