Bilberry-based food supplements

Vaccinium myrtillus L. - Bilberry (wild European blueberry)
© IPBP
Vaccinium corymbosum L. - Highbush blueberry (Cultivated blueberry)
© IPBP

Bilberries (Vaccinium myrtillus L., Ericaceae) are not only a popular wild fruit but are also used medicinally, e.g. for the symptomatic treatment of mild diarrhoea and to relieve a feeling of heaviness in the legs in cases of mild venous circulatory disorders. The black-blue wild fruits are an expensive commercial product and should not be mixed up with cultivated blueberries (Highbush blueberries, V. corymbosum L.), which show a different profile of secondary plant metabolites. Bilberries are characterised by anthocyanins, which are also responsible for the characteristic colour of the fruits.

In the present study, 11 food supplements were examined. Fresh and dried fruits as well as juices were also analysed for comparison. The following two quality markers were checked for all products (in addition to other parameters 1):

  •     Authenticity (HPLC / UV detection, fingerprint chromatograms)
  •     Total anthocyanin content (HPLC / UV detection)

Instrument parameters, analytical conditions and sample chromatograms 1

Conclusion: While the majority of the fruits and juices analysed were of good quality (see publications below), serious quality deficits were found in the segment of food supplements. Almost half of all food supplements examined had to be certified as being of unacceptable quality.

Assessment of the products (incl. illustrations2) The results obtained can be divided into the following categories (colours according to the traffic light principle).

Category 1: Products which fully comply with the declaration or which can be certified as being of very good quality overall (= high anthocyanin content).

Category 2: Products with low anthocyanin content, not recommended

Category 3: Products whose anthocyanin content deviates very strongly from the declaration.

Category 4: Products with an atypical anthocyanin pattern (= authenticity does not correspond !)

Category 5: Products without detectable anthocyanin content


Notes:
1 For details see publications [3] and [4].
2 Click on the images to enlarge them.
#01 (#23) Blueantox® body
01b
© IPBP/Gaspar

Declaration
Composition: 200 mg bilberry extract per capsule.
Also contains: vitamin B1, B2, B6, B12, nettle extract, magnesium citrate + oxide, microcrystalline cellulose, rice extract.
Recommended intake: 4 capsules / day

Results:
Authenticity: HPLC fingerprint corresponds to V. myrtillus
Declared/analysed anthocyanin content per capsule: not specified /58.6 mg

Assessment:

Category 1

#02 (#24) Blueantox® Sport Edition
02b
© IPBP/Gaspar

Declaration
Composition: 200 mg bilberry extract per capsule
Also contains: Cordyceps sinensis powder, magnesium citrate + oxide, nettle extract, vitamins B1, B2, B6, B12, rice extract, microcrystalline cellulose
Recommended intake: 4 capsules / day.

Results:
Authenticity: HPLC fingerprint cromatogram corresponds to V. myrtillus
Declared/analysed anthocyanin content per capsule: not specified /53.9 mg

Assessment:

Category 1

#03 (#25) Blueantox® Nature
03b
© IPBP/Gaspar

Declaration
Composition: 200 mg bilberry extract per capsule
Also contains: Mg citrate + oxide, vitamins B1, B2, B6, B12, rice extract, microcrystalline cellulose
Recommended intake: 2 capsules / day

Results:
Authenticity: HPLC fingerprint chromatogram corresponds to V. myrtillus
Declared/analysed anthocyanin content per capsule: not specified /53.5 mg

Assessment:

Category 1

#04 (#26) Myrtillus plus Kapseln
04b
© IPBP/Gaspar

Declaration
Composition: 100 mg bilberry extract (45:1) per capsule, of which 10 mg anthocyanins
Also contains: Tagetes erecta extract (19.9 %), bilberry leaf powder (16.5 %), zinc gluconate, tocopheryl acetate, copper gluconate, excipients
Recommended intake: 1 capsule / day.

Results:
Authenticity: HPLC fingerprint chromatogram does not correspond to V. myrtillus, anthocyanin pattern corresponds to that of "black rice"
Declared/analysed anthocyanin content per capsule: 10 mg / 0.2 mg

Assessment:

Category 3/4

#05 (#27) Heidelbeeren Myrtillus Kapseln
05b
© IPBP/Gaspar

Declaration
Composition: 200 mg bilberry concentrate per capsule
Also contains: aronia berry extract, vitamin C, B6, B12, folic acid, excipients
Recommended intake: 3 capsules / day

Results:
Authenticity: HPLC fingerprintchromatogram corresponds to V. myrtillus (but only very low peak intensity).
Declared/analysed anthocyanin content per capsule: 156 mg / 0.3 mg

Assessment:

Category 3

#06 (#28) Heidelbeere Plus Kapseln
06b
© IPBP/Gaspar

Declaration
Composition: 400 mg bilberry powder + 20 mg bilberry extract per capsule, 19 mg "anthocyanodins "* / capsule
(*designation unclear). Also contains: zinc gluconate, cellulose
Recommended intake: 2 capsules / day

Results:
Authenticity: HPLC fingerprint does not correspond to V. myrtillus, anthocyanin pattern corresponds to that of "black rice"
Declared/analysed anthocyanin content per capsule: 19 mg / 5.3 mg

Assessment:

Category 3/4

#07 (#29) Heidelbeer-Extrakt mit Anthocyanen
07b
© IPBP/Gaspar

Declaration
Composition: Bilberry extract 500 mg (of which anthocyanins 125 mg) per capsule
Also contains: leucine, calcium salts of orthophosphoric acid, hydroxypropyl methylcellulose (capsule shell), microcrystalline cellulose
Recommended intake: 3 capsules / day.

Results:
Authenticity: no anthocyanins detectable in the HPLC fingerprint chromatograms
Declared/analysed anthocyanin content per capsule: 125 mg / not detectable

Assessment:

Category 5

#08 (#30) Heidelbeer Extrakt 80 mg
08b
© IPBP/Gaspar

Declaration
Composition: 80 mg (Vaccinium myrtillus) extract per capsule, of which 20 mg "anthocyanosides "*.
(*Note: term is outdated)
Also contains: microcrystalline cellulose, ascorbyl palmitate
Recommended intake: 3 capsules / day

Results:
Authenticity: HPLC fingerprint chromatogram corresponds to V. myrtillus
Declared/analysed anthocyanin content per capsule: 20 mg / 21.0 mg

Assessment:
Note: declared and analysed anthocyanin content match.

Category 1

#09 (#31) Heidelbeer P 400 mg GPH Kapseln
09b
© IPBP/Gaspar

Declaration
Composition: 400 mg bilberry powder per capsule.
Contains additionally: maltodextrin, capsule shell (gelatine) and colouring iron oxide
Recommended intake: 3 capsules / day

Results:
Authenticity: HPLC fingerprint chromatogram corresponds to V. myrtillus
Declared/analysed anthocyanin content per capsule: not specified / 0.6 mg

Assessment:
Note: anthocyanin content (0.6 mg/capsule) is very low compared to other products (20-60 mg/capsule) (►no extract but dried fruit powder).

Category 2

#10 (#32) Standardized European Bilberry Extract
10b
© IPBP/Gaspar

Declaration
Composition: 100 mg MirtoSelect® European bilberry extract (fruits) per capsule, stand. to 36% total anthocyanins
Contains additionally: rice flour, cellulose (capsule), L-leucine, silica
Recommended intake: 1 capsule / day.

Results:
Authenticity: HPLC fingerprint chromatogram corresponds to V. myrtillus
Declared/analysed anthocyanin content per capsule: 36 mg / 36.3 mg

Assessment:
Note: declared and analysed anthocyanin content match.

Category 1

#11 (#33) Bilberry Extract (Vaccinium myrtillus)
11b
© IPBP/Gaspar

Declaration
Composition: 250 mg extract Bilberry (V. myrtillus fruit, 4 :1), equivalent to 1000 mg fruit
Also contains: gelatine (capsule), cellulose, silica, vegetable stearate
Recommended intake: 1 capsule / day

Results:
Authenticity: HPLC fingerprint chromatogram does not correspond to V. myrtillus, chromatogram shows only 2 anthocyanins and anthocyanidins instead of the usual 20
Declared/analysed anthocyanin content per capsule: not specified / 2.1 mg

Assessment:

Category 4