Dr. Verena Spiegler
© Uni MS/Dziemba

Dr. Verena Spiegler

Institute for Pharmaceutical Biology and Phytochemistry, Corrensstraße 48, 48149 Münster

Tel.: +49 251 83 33376

E-Mail: Address   Room: BC 120.282

© Uni MS/IPBP

Research

• Bioactivity of plant extracts and natural products

Investigation of bioactivities and mode of action for selected plant extracts and natural products in nematodes, microorganisms and human cell lines using different in vitro assays and methods of molecular and cell biology.

• Isolation and structure elucidation of plant secondary metabolites. Bioassay-guided fractionation.

Preparative phytochemistry, including different chromatographic methods: CC, MPLC, FCPC, HPLC. Analytical methods: UHPLC-PDA / -FLR / -MS, UHPLC-qTOF-MS, GC-MS, DC, Polarimetry, NMR

Current projects

• Anthelmintic activities of proanthocyanidins

• Natural products against virulence factors of Pseudomonas aeruginosa

• Structure elucidation and bioactivity of indole alkaloids isolated from traditionally used plant extracts

Teaching

• Praktikum Pharmazeutische Biologie III (Biologische und Phytochemische Untersuchungen) für Pharmazeuten

  • Honors

    Phytotherapie-Preis zur Förderung des wissenschaftlichen Nachwuchses – Gesellschaft für Phytotherapie (GPT)

  • Publications

    Articles

    Articles in Scientific Journals, Newspapers or Magazines
    Research Articles (Journals)
    • Grimm, Manuel; Börner, Ramona; Addotey, John N.; Schmidt, Thomas J.; Spiegler, Verena. . ‘Alstoboonine, an Ulean-Type Indole Alkaloid from Alstonia boonei Leaves.’ Journal of Natural Products 87: 514–519. doi: 10.1021/acs.jnatprod.3c00832.
    • Jacobtorweihen, Joshua; Obaidi, Ismael; Sherlock, Lee; Sheridan, Helen; Spiegler, Verena. . ‘A new lanosyl guanidine from Vertebrata lanosa with anti-inflammatory activity.’ Natural Product Research 11: 1–7. doi: 10.1080/14786419.2024.2301759.
    • Apolinário Chibli, Lucas; Ribeiro de Lima, Bruna; Magalhães Carneiro, Ariadne; Cardoso Figueiró, Harley; Cicek, Serhat Sezai; Spiegler, Verena; Soleo Funari, Cristiano. . ‘Toward a More Sustainable Sample Preparation in Phytochemistry: Case Studies in Four Subclasses of Alkaloids.’ Journal of Natural Products 87: 470–479. doi: 10.1021/acs.jnatprod.3c01048.
    • Belga, François Ngnodandi; Waindok, Patrick; Raulf, Marie-Kristin; Jato, Jonathan; Orman, Emmanuel; Rehbein, Steffen; Spiegler, Verena; Liebau, Eva; Hensel, Andreas; Ndjonka, Dieudonné; Strube, Christina. . ‘Phytochemical analysis and anthelmintic activity of Combretum mucronatum leaf extract against infective larvae of soil-transmitted helminths including ruminant gastrointestinal nematodes.’ Parasites and Vectors 17: 99. doi: 10.1186/s13071-024-06194-9.
    • Jacobtorweihen, Joshua; Hartmann, Anja; Hofer, Stefanie; Spiegler, Verena. . ‘Antibacterial Activities of the Algal Bromophenol Methylrhodomelol Against Pseudomonas aeruginosa.’ Planta Medica 90, No. 06: 469–481. doi: 10.1055/a-2289-2423.
    • Jato, Jonathan; Orman, Emmanuel; Boakye, Yaw Duah; Belga, François Ngnodandi, Ndjonka, Dieudonné; Oppong Bekoe, Emelia; Liebau, Eva; Spiegler, Verena; Hensel, Andreas; Agyare, Christian. . ‘Influence of fecal fermentation on the anthelmintic activity of proanthocyanidins and ellagitannins against human intestinal nematodes and Caenorhabditis elegans. .’ Frontiers in Pharmacology 15: 1390500. doi: 10.3389/fphar.2024.1390500.
    • Belga, François Ngnodandi; Raulf Marie-Kristin; Spiegler, Verena; Liebau, Eva; Hensel, Andreas; Strube, Christina; Ndjonka, Dieudonné. . ‘In vivo anthelmintic activity of Combretum mucronatum Schumach & Thonn leaf extract against Haemonchus contortus in goats.’ Veterinary Parasitology 331: 110288.
    • Orman, Emmanuel; Bekoe, Samuel Oppong; Asare-Nkansah, Samuel; Kralisch, Ina; Jato, Jonathan; Spiegler, Verena; Agyare, Christian; Bekoe, Emelia Oppong; Hensel, Andreas. . ‘Development of an analytical workflow to support the establishment of monographs in African pharmacopoeias – Combretum mucronatum leaves as example.’ Planta Medica 89, No. 09: 860–878. doi: 10.1055/a-2002-2260.
    • Jato, Jonathan; Waindok, Patrick; Belga François Ngnodandi, François Ngnodandi; Orman, Emmanuel; Agyare, Christian; Oppong Bekoe, Emelia; Strube, Christina; Hensel, Andreas; Liebau, Eva; Spiegler, Verena  . . ‘Anthelmintic Activities of Extract and Ellagitannins from Phyllanthus urinaria against Caenorhabditis elegans and Zoonotic or Animal Parasitic Nematodes.’ Planta Medica 89: 1215–1228. doi: 10.1055/a-2117-9426.
    • Orman, Emmanuel; Kralisch, Ina; Jato, Jonathan; Agyare, Christian; Spiegler, Verena; Oppong Bekoe, Emelia; Hensel, Andreas. . ‘Towards the Development of Analytical Monograph Specifications for the Quality Assessment of the Medicinal Plant Phyllanthus urinaria.’ Phytochemistry 215. doi: 10.1016/j.phytochem.2023.113854.
    • Jacobtorweihen, Joshua; Schmitt, Marthe; Spiegler, Verena. . ‘Amino Acid-Coupled Bromophenols and a Sulfated Dimethylsulfonium Lanosol from the Red Alga Vertebrata lanosa.’ Marine Drugs 20, No. 7. doi: 10.3390/md20070420.
    • Codo Toafode, Nonvignon Murielle; Marquardt, Peter; Ahyi, Virgile; Fester, Karin; Spiegler, Verena; Vissiennon, Cica. . ‘Anti-Inflammatory Potential of Phenolic Compounds Isolated From Entada africana Guill. & Perr. Used in the Republic of Benin.’ Frontiers in Pharmacology 13. doi: 10.3389/fphar.2022.931240.
    • Hake, Alexander; Begrow, Frank; Spiegler, Verena; Symma, Nico; Hensel Andreas; Düfer, Martina. . ‘Effects of Extracts and Flavonoids from Drosera rotundifolia L. on Ciliary Beat Frequency and Murine Airway Smooth Muscle.’ Molecules 27: 6622. doi: 10.3390/molecules27196622.
    • Greiffer, Luise; Liebau, Eva; Herrmann, Fabian C.; Spiegler, Verena. . ‘Condensed tannins act as anthelmintics by increasing the rigidity of the nematode cuticle.’ Scientific Reports 12: 18850. doi: 10.1038/s41598-022-23566-2.
    • Baumeister A, Gardemann J, Fobker M, Spiegler V, Fischer T. . ‘Short-Term Influence of Caffeine and Medium-Chain Triglycerides on Ketogenesis: A Controlled Double-Blind Intervention Study.’ Journal of Nutrition and Metabolism 2021. doi: 10.1155/2021/1861567.
    • Spiegler V, Greiffer L, Jacobtorweihen J, Asase A, Lanvers-Kaminsky C, Hempel G, Agyare C, Hensel A. . ‘In vitro screening of plant extracts traditionally used as cancer remedies in Ghana - 15-Hydroxyangustilobine A as the active principle in Alstonia boonei leaves.’ Journal of Ethnopharmacology 265. doi: 10.1016/j.jep.2020.113359.
    • Spiegler V. . ‘Anthelmintic A-Type Procyanidins and Further Characterization of the Phenolic Composition of a Root Extract from Paullinia pinnata.’ Molecules 25, No. 10. doi: 10.3390/molecules25102287.
    • Spiegler V, Gierlikowska B, Saenger T, Addotey JN, Sendker J, Jose J, Kiss AK, Hensel A. . ‘Root Extracts From Ononis spinosa Inhibit IL-8 Release via Interactions With Toll-Like Receptor 4 and Lipopolysaccharide.’ Frontiers in Pharmacology 2020. doi: 10.3389/fphar.2020.00889.
    • Hensel A, Bauer R, Heinrich M, Spiegler V, Kayser O, Hempel G, Kraft K. . ‘Challenges at the Time of COVID-19: Opportunities and Innovations in Antivirals From Nature.’ Planta Medica 86. doi: 10.1055/a-1177-4396.
    • Hensel A, Spiegler V, Kraft K. . „Pflanzliche Extrakte gegen virale Infektionen des oberen Rachenraumes.“ Zeitschrift für Phytotherapie 41, No. 02: 52–54. doi: 10.1055/a-1102-1662.
    • Lehmann S, Herrmann F, Kleemann K, Spiegler V, Liebau E, Hensel A. . ‘Extract and the quassinoid ailanthone from Ailanthus altissima inhibit nematode reproduction by damaging germ cells and rachis in the model organism Caenorhabditis elegans.’ Fitoterapia 146. doi: 10.1016/j.fitote.2020/104651.
    • Herrmann FC, Spiegler V. . ‘Caenorhabditis elegans revisited by atomic force microscopy - Ultra-structural changes of the cuticle, but not in the intestine after treatment with Combretum mucronatum extract.’ Journal of Structural Biology 208: 174–181. doi: 10.1016/j.jsb.2019.08.013.
    • Grube K, Spiegler V, Hensel A. . ‘Antiadhesive phthalides from Apium graveolens fruits against uropathogenic E. coli.’ Journal of Ethnopharmacology 237: 300–306. doi: 10.1016/j.jep.2019.03.024.
    • Agyare C, Spiegler V, Asase A, Scholz M, Hempel G, Hensel A. . ‘An ethnopharmacological survey of medicinal plants traditionally used for cancer treatment in the Ashanti region, Ghana.’ Journal of Ethnopharmacology 212: 137–152. doi: 10.1016/j.jep.2017.10.019.
    • Spiegler V., Hensel A., Seggewiß J., Lubisch M., Liebau E. . ‘Transcriptome analysis reveals molecular anthelmintic effects of procyanidins in C. elegans.’ PloS one 12, No. 9. doi: 10.1371/journal.pone.0184656.
    • Spiegler V., Liebau E., Peppler C., Raue K., Werne S., Strube C., Heckendorn F., Agyare C., Stark T., Hofmann T., Hensel A. . ‘A Hydroalcoholic Extract from Paullinia pinnata LRoots Exerts Anthelmintic Activity against Free-Living and Parasitic Nematodes.’ Planta Medica 82, No. 13: 1173–1179. doi: 10.1055/s-0042-108209.
    • Spiegler V, Sendker J, Petereit F, Liebau E, Hensel A. . ‘Bioassay-Guided Fractionation of a Leaf Extract from Combretum mucronatum with Anthelmintic Activity: Oligomeric Procyanidins as the Active Principle.’ Molecules 20, No. 8: 14810–14832. doi: 10.3390/molecules200814810.
    • Agyare, C., Spiegler, V., Sarkodie, H., Asase, Alex A., Liebau, E., Hensel, A. . ‘An ethnopharmacological survey and in vitro confirmation of the ethnopharmacological use of medicinal plants as anthelmintic remedies in the Ashanti region, in the central part of Ghana.’ Journal of Ethnopharmacology 158, No. PA: 263. doi: 10.1016/j.jep.2014.10.029.
    Review Articles (Journals)
    • Jacobtorweihen, Joshua; Spiegler, Verena. . ‘Phylogenetic distribution of bromophenols in marine algae and the generation of a comprehensive bromophenol database.’ Phytochemistry Reviews 22: 505–554. doi: 10.1007/s11101-022-09847-8.
    • Orman, Emmanuel; Oppong Bekoe, Samuel; Jato, Jonathan; Spiegler, Verena; Asare-Nkansah, Samuel; Agyare, Christian; Hensel, Andreas; Oppong Bekoe, Emelia. . ‘Quality assessment of African herbal medicine: A systematic review and the way forward.’ Fitoterapia 162: 105287. doi: 10.1016/j.fitote.2022.105287.
    • Jato, Jonathan; Orman, Emmanuel; Duah Boakye, Yaw; Oppong Bekoe, Emelia; Oppong Bekoe, Samuel; Asare-Nkansah, Samuel, Spiegler, Verena; Hensel, Andreas; Liebau, Eva; Agyare, Christian. . ‘Anthelmintic Agents from African Medicinal Plants: Review and Prospects.’ Evidence-Based Complementary and Alternative Medicine 2022: 8023866. doi: 10.1155/2022/8023866.
    • Spiegler Verena; Liebau Eva; Hensel Andreas. . ‘Medicinal plant extracts and plant-derived polyphenols with anthelmintic activity against intestinal nematodes.’ Natural Product Reports 34, No. 6: 627–643. doi: 10.1039/c6np00126b.
    Conference Contributions
    Abstracts in Journals (Conferences)
    • Orman, Emmanuel; Jato, Jonathan; Oppong Bekoe, Emelia; Oppong Bekoe, Samuel; Asare-Nkansah, Samuel; Spiegler, Verena; Hensel, Andreas. . ‘Analytical investigations reveal challenges with the collection of Combretum mucronatum (Schumach. & Thonn.) leaves for herbal medicine purposes in Ghana.’ Contributed to the Annual Meeting of the Society for Medicinal Plant and Natural Product Research 2022, Thessaloniki. doi: 10.1055/s-0042-1758901.
    • Jacobtorweihen, Joshua; Spiegler, Verena. . ‘The establishment of an analytical database of algal bromophenols and phytochemical characterization of the red alga Vertebrata lanosa.’ Contributed to the Annual Meeting of the Society for Medicinal Plant and Natural Product Research 2022, Thessaloniki. doi: 10.1055/s-0042-1758883.
    • Spiegler, Verena. . „Anthelminthische Aktivität von Gerbstoffdrogen und tanninreichen Extrakten bei intestinalen Parasiten.“ Beitrag präsentiert auf der Phytokongress 2021, Bonn. doi: 10.1055/s-0041-1732835.
    • Greiffer, Luise; Herrmann, Fabian; Spiegler, Verena. . „Ultrastrukturelle Untersuchungen an Caenorhabditis elegans nach Behandlung mit Combretum mucronatum Extrakt.“ Beitrag präsentiert auf der Phytokongress 2021, Bonn. doi: 10.1055/s-0041-1731470.
    • Spiegler, Verena; Michalak, Barbara; Addotey, John; Saenger, Thorsten; Jose Joachim; Kiss Anna K.; Hensel, Andreas. . ‘Root extracts from Ononis spinosa exert anti-inflammatory activity in vitro on IL-8 and TNF-α release by inhibition of TLR-4 receptor.’ Contributed to the Annual Meeting of the Society for Medicinal Plant and Natural Product Research 2019, Innsbruck. doi: 10.1055/s-0039-3399691.
    • Hensel, Andreas; Sarshar, Shabnam; Gottesmann, Maren; Deipenbrock, Melanie; Spiegler, Verena. . ‘New weapons against bacterial adhesion, invasion, quorum sensing, cell damaging facors of pathogens: Natural products as new anti-virulence compounds.’ Contributed to the . Annual Meeting of the Society for Medicinal Plant and Natural Product Research 2019, Innsbruck. doi: 10.1055/s-0039-3399719.
    • Spiegler, Verena; Michalak, Barbara; Addotey, John; Saenger, Thorsten; Jose, Joachim; Kiss, Anna K.; Hensel, Andreas. . „Antiinflammatori-sche Wurzelextrakte aus Ononis spinosa verringern die Freisetzung von IL-8 und TNF-α in vitro durch Antagonismus am TLR4 Rezeptor.“ Beitrag präsentiert auf der Phytokongress 2019, Münster. doi: 10.1055/s-0039-1697294.
    • Spiegler, Verena; Grube, Kim; Scharf, Birte; Sarshar, Shabnam; Hensel, Andreas. . ‘Phthalide aus Apiaceendrogen als antiadhäsive Naturstoffe gegen uropathogene E. coli.’ Contributed to the Phytokongress 2019, Münster. doi: 10.1055/s-0039-1697284.
    • Spiegler, Verena; Liebau, Eva; Hensel, Andreas. . ‘Anthelmintic activity of procyanidins from West African medicinal plants – Insights into phytochemistry and molecular targets.’ Contributed to the Annual Meeting of the Society for Medicinal Plant and Natural Product Research 2017, Basel. doi: 10.1055/s-0037-1608560.
    • Spiegler, Verena; Liebau, Eva; Hensel, Andreas. . „Molekularbiologische Untersuchung anthelminthischer Effekte von Procyanidinen auf C. elegans.“ Beitrag präsentiert auf der Phytokongress 2017, Münster. doi: 10.1055/s-0037-1607134.
    • Spiegler Verena; Raue, Katharina; Strube, Christina; Liebau, Eva; Hensel, Andreas. . ‘Anthelmintic effects of tannin-rich plants and oligomeric proanthocyanidin clusters against parasitic and free-living nematodes.’ Contributed to the Annual Meeting of the Society of Medicinal Plant and Natural Product Research 2015, Budapest. doi: 10.1055/s-0035-1565347.
    Posters
    • Greiffer, Luise; Herrmann, Fabian C.; Spiegler, Verena. ‘Anthelmintic activities of procyanidins targeting the cuticle of the nematode Caenorhabditis elegans – microscopic observations and the impact of interflavan-linkages.’ contributed to the Annual Meeting of the Society for Medicinal Plant and Natural Product Research 2022, Thessaloniki, . doi: 10.1055/s-0042-1759243.
    • Börner, Ramona; Grimm, Manuel; Greiffer, Luise; Jacobtorweihen, Joshua; Spiegler, Verena. ‘Cytotoxic activities of 15-hydroxyangustilobine A, a major indole alkaloid from the leaves of Alstonia boonei.’ contributed to the Annual Meeting of the Society for Medicinal Plant and Natural Product Research 2022, Thessaloniki, . doi: 10.1055/s-0042-1759244.
    • Jacobtorweihen, Joshua; Hartmann, Anja; Hofer, Stefanie; Spiegler, Verena. ‘Assessment of the antimicrobial and antivirulent properties of the marine bromophenol methylrhodomelol against Pseudomonas aeruginosa.’ contributed to the Annual Meeting of the Society for Medicinal Plant and Natural Product Research 2022, Thessaloniki, . doi: 10.1055/s-0042-1759004.
    • Codo Toafode, Nonvignon Murielle; Marquardt, Peter; Ahyi, Virgile; Fester, Karin; Spiegler, Verena; Vissiennon, Cica. ‘Anti-inflammatory potential of phenolic compounds isolated from Entada africana Guill. & Perr. used in the Republic of Benin.’ contributed to the Annual Meeting of the Society for Medicinal Plant and Natural Product Research 2022, Thessaloniki, . doi: 10.1055/s-0042-1759125.
    • Codo Toafode M, Spiegler V, Vissiennon C, Ahyi V, Fester K. ‘In vitro anti-inflammatory potential and chemical profiling of Entada africana from the Republic of Benin.’ contributed to the Annual Meeting of the Society for Medicinal Plant and Natural Product Research 2021, Virtual Conference, Bonn, . doi: 10.1055/s-0041-1736987.
    • Spiegler V, Jato J, Hensel A, Orman E, Liebau E. ‘Anthelmintic potential of Phyllanthus urinaria L. (Phyllanthaceae) in the model organism Caenorhabditis elegans.’ contributed to the Annual Meeting of the Society for Medicinal Plant and Natural Product Research 2021, Virtual Conference, Bonn, . doi: 10.1055/s-0041-1736821.
    • Jacobtorweihen J, Spiegler V. ‘Die Zinkhomöostase als Target für virulenzmindernde Wirkstoffe? – Ein Proliferationsscreening an Pseudomonas aeruginosa.’ präsentiert auf der Phytokongress 2021, Bonn (Online), . doi: 10.1055/s-0041-1731474.
    • Greiffer L, Herrmann F, Spiegler V. ‘Ultrastructural studies on Caenorhabditis elegans after treatment with Combretum mucronatum extract.’ contributed to the Junior Zoonoses Meeting, Online-Symposium, .
    • Greiffer L, Spiegler V, Jacobtorweihen J, Hensel A. ‘Zytotoxische Effekte eines traditionell verwendeten Extraktes aus Alstonia boonei Blättern.’ präsentiert auf der Phytokongress 2019, Münster, . doi: 10.1055/s-0039-1697300.
    • Hensel A., Grube K., Scharf B., Spiegler V. ‘Antiadhesive activity of the phthalide sedanenolide from Apium graveolens fruits (celery) against uropathogenic E. coli.’ contributed to the Annual Meeting of the Society for Medicinal Plant and Natural Product Research 2019, Innsbruck, . doi: 10.1055/s-0039-3399950.
    • Spiegler V. ‘Coumarins and anthelmintic A-type procyanidins from the roots of Paullinia pinnata L.’ contributed to the Annual Meeting of the Society for Medicinal Plant and Natural Product Research 2019, Innsbruck, . doi: 10.1055/s-0039-3400136.
    • Spiegler V, Jacobtorweihen J, Agyare C, Hensel A. ‘In vitro cytotoxic effects of a traditionally used extract from the bark of Kalanchoe integra.’ contributed to the Annual Meeting of the Society for Medicinal Plant and Natural Product Research 2018, Shanghai, .
    • Spiegler V, Lubisch M, Hensel A, Liebau E. ‘The nematode strikes back – C. elegans’ proline-rich response to treatment with oligomeric procyanidins.’ contributed to the Annual Meeting of the Society for Medicinal Plant and Natural Product Research 2017, Basel, . doi: 10.1055/s-0037-1608552.
    • Spiegler V, Peppler C, Werne S, Heckendorn F, Sendker J, Liebau E, Agyare C, Hensel A. ‘Anthelmintic activity of a traditionally used root extract from Paullinia pinnata.’ contributed to the Annual Meeting of the Society for Medicinal Plant and Natural Product Research 2016, Copenhagen, . doi: 10.1055/s-0036-1596924.
    • Spiegler V, Peppler C, Raue K, Werne S, Strube C, Heckendorn F, Liebau E, Hensel A. ‘Anthelminthische Wirkung eines hydroethanolischen Extrakts aus Weißdornblättern.’ präsentiert auf der Phytokongress 2016, Bonn, . doi: 10.1055/s-0036-1584425.

    Theses (Doctoral or Postdoctoral)

    • Spiegler, Verena. . Anthelmintic activity of procyanidins from West African plants. From traditional medicine to phytochemistry and molecular investigations. Dissertation thesis, Universität Münster.