Food supplements based on D-mannose

In the present study, seven mannose preparations from the trade were investigated. The quantification of the mannose content was carried out with a specific chromatographic method: HPAEC-PAD.

Instrument parameters, analytical conditions and example chromatograms

All samples were additionally analysed qualitatively by U(H)PLC-ESI/qTOF. The results (base peak chromatograms, "BPC") are shown below as a stacked plot (analysis conditions: on request).

Conclusion

The quality of all mannose-containing samples analysed was satisfactory with respect to the content of the declared ingredient D-mannose and not objectionable. The results were published in a scientific journal: DOI: 10.1055/a-1857-9517

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Figure 1. D-mannose. In aqueous solution, an equilibrium of the two anomeric pyranose forms is established (approx. 65% α- and 35% β-form). The two anomeric furanose forms (not shown) and the open-chain structure are detectable only in traces.

The following products were analysed:

Note: The chromatograms open by clicking.
#1 sanotact® Mannose
01
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Medical device
Composition: D-mannose (from maize), hydroxypropylmethyl cellulose, microcrystalline cellulose, magnesium stearate.
Dosage form: Tablet - 500 mg

#2 dm Mivolis Mannose
 02
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Medical device
Composition: D-mannose
Dosage form: Stick (sachet) - 2000 mg

#3 Manosan ®
 03
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Medicinal device
Composition: D-mannose, microcrystalline cellulose, sorbitol, tricalcium phosphate, polyethylene glycol, magnesium salts of fatty acids.
Dosage form: Tablet - 1000 mg
 

#4 Femannose ® N
 04
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Medical device
Composition: D-mannose, fructose, sucralose, citric acid, beetroot food colouring, silicon dioxide, flavouring.
Dosage form: sachet - 2000 mg

#5 tetesept: Femi Blase ®
 05
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Medical device
Composition: D-mannose, dextrose, sorbitol, inulin, tricalcium phosphate, cranberry, magnesium stearate, stevia.
Dosage form: sachet - 2000 mg
 

#6 Natural D-Mannose - Naturally made from birch
 06
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Food supplement
Composition: D-Mannose
Dosage form: powder

#7 D-Mannose - Without additives
 07
© Lechtenberg

Food supplement
Composition: D-Mannose
Dosage form: powder

U(H)PLC - qTOF Analysis
Stack Qtof
BPC: m/z 200 - m/z 1000
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U(H)PLC - qTOF Analysis

Extraction solvent: MeOH 50% (= methanol-H2O, 50/50, v/v)

Extraction: 500 mg in 10 mL, ultrasonic bath, centrifugation, filtration

Dilution: before injection 1:1 (with MeOH 50%)