Improving intravital microscopy of inflammatory cell response by active motion compensation with controlled adaptive optics
Principal investigators: Thomas Huser, Benedikt Wirth
Project number: CRC 1450 A06
Project term: 01/2021–12/2024
We will advance multiphoton fluorescence microscopy by developing a novel optical module comprised of a high-speed deformable mirror that will actively compensate tissue motion during intravital imaging (1, 2), for instance due to heart beat (8 Hz), breathing (3 Hz, in mm-range) or peristaltic movement of the gut in mice. To control this module in real-time, we will develop and validate mathematical methods (3, 4) that track and predict tissue deformation.
This will allow imaging of inflammatory processes at cellular resolution without mechanical tissue fixation (5).
Team
Principal investigators
- Huser, Thomas, Univ.-Prof. Dr. phil. II
Faculty of Physics, Bielefeld University - Wirth, Benedikt, Univ.-Prof. Dr. rer. nat.
Applied Mathematics: Institute for Analysis and Numerics
Project members
- Beutler, Sascha
Applied Mathematics: Institute for Analysis and Numerics - Kunisch, Manuel
Faculty of Physics, Bielefeld University - Pilger, Christian, Dr. rer. nat.
Faculty of Physics, Bielefeld University
Publications
The names of the principal investigators in our network have been bolded. Publications released prior to 2021, when funding for our network commenced, represent previous project-related work.
2024
Wen J, Pilger C, Wang W, Erapaneedi R, Xiu H, Fan Y, Hu X, Huser T, Kiefer F, Wei X, Yang Z. Watt-level all polarization-maintaining femtosecond fiber laser source at 1100 nm. Opt. Express 2024;32: 9625. Abstract |
2023
Guastini M, Rajković M, Rumpf M, Wirth B. The Variational Approach to the Flow of Sobolev-Diffeomorphisms Model. Scale Space and Variational Methods in Computer Vision 2023: 551-564. Abstract |
Kong C, Pilger C, Kunisch M, Förster C, Schulte am Esch J, Huser T. Hyperspectral Coherent Raman Scattering (CRS) Microscopy Based on a Rapidly Tunable and Environmentally Stable Fiber Laser. Laser & Photonics Reviews 2023;17Abstract |
Ortkrass H, Schurstedt J, Wiebusch G, Szafranska K, McCourt P, Huser T. High-speed TIRF and 2D super-resolution structured illumination microscopy with a large field of view based on fiber optic components. Opt Express 2023;31: 29156-29165. Abstract |
2022
Sandmeyer A, Wang L, Hubner W, Muller M, Chen BK, Huser T. Cost-effective high-speed, three-dimensional live-cell imaging of HIV-1 transfer at the T cell virological synapse. iScience 2022;25: 105468. Abstract |
Striewski P, Wirth B. Elastic 3D–2D Image Registration. J Math Imaging Vis 2022;64: 443-462. Abstract |
2021
Effland A, Heeren B, Rumpf M, Wirth B. Consistent curvature approximation on Riemannian shape spaces 2021;42: 78-106. Abstract |
Hardering H, Wirth B. Quartic Lp-convergence of cubic Riemannian splines. IMA Journal of Numerical Analysis 2021Abstract |
Kong C, Bobe S, Pilger C, Lachetta M, Oie CI, Kirschnick N, Monkemoller V, Hubner W, Forster C, Schuttpelz M, Kiefer F, Huser T, Schulte Am Esch J. Multiscale and Multimodal Optical Imaging of the Ultrastructure of Human Liver Biopsies. Front Physiol 2021;12: 637136. Abstract |
Sandmeyer A, Lachetta M, Sandmeyer H, Hübner W, Huser T, Müller M. Cost-Effective Live Cell Structured Illumination Microscopy with Video-Rate Imaging. ACS Photonics 2021;8: 1639-1648. Abstract |
2019
Heeren B, Rumpf M, Wirth B. Variational time discretization of Riemannian splines. IMA Journal of Numerical Analysis 2019;39: 61–104. Abstract |
Markwirth A, Lachetta M, Monkemoller V, Heintzmann R, Hubner W, Huser T, Muller M. Video-rate multi-color structured illumination microscopy with simultaneous real-time reconstruction. Nat Commun 2019;10: 4315. Abstract |
2017
Berkels B, Wirth B. Joint denoising and distortion correction of atomic scale scanning transmission electron microscopy images. Inverse Problems 2017;33: 095002. Abstract |
Song J, Zhang X, Buscher K, Wang Y, Wang H, Di Russo J, Li L, Lutke-Enking S, Zarbock A, Stadtmann A, Striewski P, Wirth B, Kuzmanov I, Wiendl H, Schulte D, Vestweber D, Sorokin L. Endothelial Basement Membrane Laminin 511 Contributes to Endothelial Junctional Tightness and Thereby Inhibits Leukocyte Transmigration. Cell Rep 2017;18: 1256-1269. Abstract |