Improving intravital microscopy of inflammatory cell response by active motion compensation with controlled adaptive optics

Principal investigators: Thomas Huser, Benedikt Wirth
Project number: CRC 1450 A06
Project term: 01/2021–12/2024

Graphical abstract
© CRC inSight

We will advance multiphoton fluorescence microscopy by developing a novel optical module comprised of a high-speed deformable mirror that will actively compensate tissue motion during intravital imaging (1, 2), for instance due to heart beat (8 Hz), breathing (3 Hz, in mm-range) or peristaltic movement of the gut in mice. To control this module in real-time, we will develop and validate mathematical methods (3, 4) that track and predict tissue deformation.

This will allow imaging of inflammatory processes at cellular resolution without mechanical tissue fixation (5).

Team

Principal investigators

Project members

Publications

The names of the principal investigators in our network have been bolded. Publications released prior to 2021, when funding for our network commenced, represent previous project-related work.

2024

Wen J, Pilger C, Wang W, Erapaneedi R, Xiu H, Fan Y, Hu X, Huser T, Kiefer F, Wei X, Yang Z. Watt-level all polarization-maintaining femtosecond fiber laser source at 1100  nm. Opt. Express 2024;32: 9625. Abstract

2023

Guastini M, Rajković M, Rumpf M, Wirth B. The Variational Approach to the Flow of Sobolev-Diffeomorphisms Model. Scale Space and Variational Methods in Computer Vision 2023: 551-564. Abstract
Kong C, Pilger C, Kunisch M, Förster C, Schulte am Esch J, Huser T. Hyperspectral Coherent Raman Scattering (CRS) Microscopy Based on a Rapidly Tunable and Environmentally Stable Fiber Laser. Laser & Photonics Reviews 2023;17Abstract
Ortkrass H, Schurstedt J, Wiebusch G, Szafranska K, McCourt P, Huser T. High-speed TIRF and 2D super-resolution structured illumination microscopy with a large field of view based on fiber optic components. Opt Express 2023;31: 29156-29165. Abstract

2022

Sandmeyer A, Wang L, Hubner W, Muller M, Chen BK, Huser T. Cost-effective high-speed, three-dimensional live-cell imaging of HIV-1 transfer at the T cell virological synapse. iScience 2022;25: 105468. Abstract
Striewski P, Wirth B. Elastic 3D–2D Image Registration. J Math Imaging Vis 2022;64: 443-462. Abstract

2021

Effland A, Heeren B, Rumpf M, Wirth B. Consistent curvature approximation on Riemannian shape spaces 2021;42: 78-106. Abstract
Hardering H, Wirth B. Quartic Lp-convergence of cubic Riemannian splines. IMA Journal of Numerical Analysis 2021Abstract
Kong C, Bobe S, Pilger C, Lachetta M, Oie CI, Kirschnick N, Monkemoller V, Hubner W, Forster C, Schuttpelz M, Kiefer F, Huser T, Schulte Am Esch J. Multiscale and Multimodal Optical Imaging of the Ultrastructure of Human Liver Biopsies. Front Physiol 2021;12: 637136. Abstract
Sandmeyer A, Lachetta M, Sandmeyer H, Hübner W, Huser T, Müller M. Cost-Effective Live Cell Structured Illumination Microscopy with Video-Rate Imaging. ACS Photonics 2021;8: 1639-1648. Abstract

2019

Heeren B, Rumpf M, Wirth B. Variational time discretization of Riemannian splines. IMA Journal of Numerical Analysis 2019;39: 61–104. Abstract
Markwirth A, Lachetta M, Monkemoller V, Heintzmann R, Hubner W, Huser T, Muller M. Video-rate multi-color structured illumination microscopy with simultaneous real-time reconstruction. Nat Commun 2019;10: 4315. Abstract

2017

Berkels B, Wirth B. Joint denoising and distortion correction of atomic scale scanning transmission electron microscopy images. Inverse Problems 2017;33: 095002. Abstract
Song J, Zhang X, Buscher K, Wang Y, Wang H, Di Russo J, Li L, Lutke-Enking S, Zarbock A, Stadtmann A, Striewski P, Wirth B, Kuzmanov I, Wiendl H, Schulte D, Vestweber D, Sorokin L. Endothelial Basement Membrane Laminin 511 Contributes to Endothelial Junctional Tightness and Thereby Inhibits Leukocyte Transmigration. Cell Rep 2017;18: 1256-1269. Abstract