Targeting of S100A8/A9 for imaging of inflammatory disorders
Principal investigators: Andreas Faust, Günter Fritz
Project number: CRC 1450 A03
Project term: 01/2021–12/2024
Activated monocytes and granulocytes are key to inflammatory diseases and release the alarmin S100A8/A9 in high concentrations, promoting sterile as well as infectious inflammatory processes via TLR4 (1). We will establish a specific S100A8/A9 small molecule ligand through combination of X-ray structure analysis and chemical synthesis (2, 3). The ligand will be linked to different labels suited for PET, SPECT, fluorescent or photoacoustic imaging techniques (4).
In summary, we will create a new S100 alarmin-based imaging toolset to monitor inflammatory responses with respect to their spatial location and temporal dynamics at various scales in living organisms.
Team
Principal investigators
- Faust, Andreas, Priv.-Doz. Dr. rer. nat.
European Institute for Molecular Imaging - Fritz, Günter, PD Dr. rer. nat.
Department of Cellular Microbiology, University of Hohenheim
Project members
- Dewies, Felix
European Institute for Molecular Imaging - Hau, Jann-Louis
Department of Cellular Microbiology, University of Hohenheim
Publications
The names of the principal investigators in our network have been bolded. Publications released prior to 2021, when funding for our network commenced, represent previous project-related work.
2023
Hau J-L, Kremser H, Knogl-Tritschler S, Stefanski V, Steuber J, Fritz G. Fast IMAC purification of non-tagged S100A8/A9 (calprotectin) from Homo sapiens and Sus scrofa. Protein Expr Purif 2023;208-209: 106275. Abstract |
Roll W, Faust A, Hermann S, Schafers M. Infection Imaging: Focus on New Tracers?. J Nucl Med 2023;64: 59s-67s. Abstract |
Stegmann DP, Steuber J, Fritz G, Wojdyla JA, Sharpe ME. Fast fragment and compound screening pipeline at the Swiss Light Source. Methods Enzymol 2023;690: 235-284. Abstract |
Steiner ST, Maisuls I, Junker A, Fritz G, Faust A, Strassert CA. Concerning the photophysics of fluorophores towards tailored bioimaging compounds: a case study involving S100A9 inflammation markers. Photochem Photobiol Sci 2023;22: 2093-2104. Abstract |
2022
Honold L, Austrup M, Faust A, Konken CP, Schwegmann K, Zinnhardt B, Daniliuc CG, Haufe G, Schafers M, Kopka K, Hermann S. Towards Optimized Bioavailability of (99m)Tc-Labeled Barbiturates for Non-invasive Imaging of Matrix Metalloproteinase Activity. Mol Imaging Biol 2022;24: 434-443. Abstract |
2018
Voeller T, Faust A, Roth J, Schafers M, Vogl T, Hermann S. A Non-Peptidic S100A9 Specific Ligand for Optical Imaging of Phagocyte Activity In Vivo. Mol Imaging Biol 2018;20: 407-416. Abstract |
2013
Damo SM, Kehl-Fie TE, Sugitani N, Holt ME, Rathi S, Murphy WJ, Zhang Y, Betz C, Hench L, Fritz G, Skaar EP, Chazin WJ. Molecular basis for manganese sequestration by calprotectin and roles in the innate immune response to invading bacterial pathogens. Proc Natl Acad Sci U S A 2013;110: 3841-3846. Abstract |
2012
Koch M, Fritz G. The structure of Ca2+-loaded S100A2 at 13-A resolution. Febs J 2012;279: 1799-1810. Abstract |
2007
Ostendorp T, Leclerc E, Galichet A, Koch M, Demling N, Weigle B, Heizmann CW, Kroneck PMH, Fritz G. Structural and functional insights into RAGE activation by multimeric S100B. Embo J 2007;26: 3868-3878. Abstract |