INTERNSHIP TOPICS - INSTRUMENTAL ANALYSIS

 

CHROMATOGRAPHY

Thin Layer Chromatography

Qualitative DC for the identification of a drug mixture

Comparison of separation performance between HPTLC (high performance thin layer chromatography) and conventional DC considering different sample introduction procedures

 

HPLC

Qualitative and quantitative determination of drugs by HPLC/UV-Vis

Analysis of a drug mixture by HPLC / Evaporative Light Scattering Detector (ELSD)

 

Ion chromatography

Determination of fluoride content in fluoride tablets

 

Gas chromatography

Generation of a van-Deemter curve

Optimization of gas chromatographic separation conditions using the example of the separation of peppermint oil into its components

Qualitative and quantitative determination of fatty acid esters by GC

Comparison of an isothermal measurement with the temperature gradient measurement of a mixture of different fatty acid esters

 

Mass spectrometry

Qualitative analysis of a fatty acid ester by GC-MS coupling

 

SPECTROSCOPY/OPTICAL METHODS

UV spectroscopy

Content determination of ascorbic acid incl. determination of the absorption maximum

Two-component analysis: simultaneous determination of the content of two drugs: pyridoxine hydrochloride / nicotinamide

Identity testing using the example of the drug ibuprofen according to the European Pharmacopoeia

 

Fluorimetry

Recording of an excitation and an emission spectrum of quinine sulfate

Quantitative fluorimetric determination of the content of a quinine sulfate solution

Quantitative fluorimetric analysis of a substance from food based on its intrinsic fluorescence using standard addition techniques.

 

Infrared spectroscopy

Investigation of specific bands of selected functional groups by FT-IR

Identification of a drug/preservative substance by IR spectroscopy (ATR crystal)

Identification of a substance by IR spectroscopy (KBr compact) .

Instructions for the preparation of a KBr compact.

Identification of crystal modifications using chloramphenicol palmitate as an example (FT-IR device, group task).

NIR spectroscopy

Identity studies of excipients by NIR spectroscopy (group task).

 

Atomic absorption/atomic emission spectroscopy (AAS/AES).

Determination of Zn concentration of zinc tablets using AAS for quality control (group experiment).

Determination of the Ca concentration of mineral waters with AES

 

Polarimetry

Penumbral polarimeter.

Handling of the penumbral polarimeter.

Investigation of the concentration dependence of the specific rotation.

Photoelectric polarimeter.

Investigation of the influence of different solvents and pH-values on the specific rotation.

Identity testing using the example of the drug N-acetyl-L-cysteine according to the European Pharmacopoeia .

Determination of enantiomeric excess and enantiomeric ratio of the drug (-)-ephedrine hydrochloride .

Specific rotation and optical rotation dispersion of sucrose solution.

 

ELECTROCHEMISTRY

Potentiometry

Potentiometric precipitation titration: Argentometric titration of potassium bromide

Potentiometric titration of acids and bases:

 

Amperometry and voltametry

Bivoltametric endpoint determination of redox titrations: Iodometric content determination of sodium thiosulfate.

 

Coulometry

Classical and modified Karl Fischer method

Water determination in organic solvents.

 

Polarography - Voltammetry

Theoretical experiments:

Polarograms with an electrolyte solution containing oxygen.

Polarographic determination of copper, lead, cadmium and zinc

Polarographic determination of ascorbic acid in fruit juices

 

Capillary electrophoresis

Qualitative analysis of drugs by capillary electrophoresis (CE)