INTERNSHIP TOPICS - INSTRUMENTAL ANALYSIS
CHROMATOGRAPHY
Thin Layer Chromatography
Qualitative DC for the identification of a drug mixture
Comparison of separation performance between HPTLC (high performance thin layer chromatography) and conventional DC considering different sample introduction procedures
HPLC
Qualitative and quantitative determination of drugs by HPLC/UV-Vis
Analysis of a drug mixture by HPLC / Evaporative Light Scattering Detector (ELSD)
Ion chromatography
Determination of fluoride content in fluoride tablets
Gas chromatography
Generation of a van-Deemter curve
Optimization of gas chromatographic separation conditions using the example of the separation of peppermint oil into its components
Qualitative and quantitative determination of fatty acid esters by GC
Comparison of an isothermal measurement with the temperature gradient measurement of a mixture of different fatty acid esters
Mass spectrometry
Qualitative analysis of a fatty acid ester by GC-MS coupling
SPECTROSCOPY/OPTICAL METHODS
UV spectroscopy
Content determination of ascorbic acid incl. determination of the absorption maximum
Two-component analysis: simultaneous determination of the content of two drugs: pyridoxine hydrochloride / nicotinamide
Identity testing using the example of the drug ibuprofen according to the European Pharmacopoeia
Fluorimetry
Recording of an excitation and an emission spectrum of quinine sulfate
Quantitative fluorimetric determination of the content of a quinine sulfate solution
Quantitative fluorimetric analysis of a substance from food based on its intrinsic fluorescence using standard addition techniques.
Infrared spectroscopy
Investigation of specific bands of selected functional groups by FT-IR
Identification of a drug/preservative substance by IR spectroscopy (ATR crystal)
Identification of a substance by IR spectroscopy (KBr compact) .
Instructions for the preparation of a KBr compact.
Identification of crystal modifications using chloramphenicol palmitate as an example (FT-IR device, group task).
NIR spectroscopy
Identity studies of excipients by NIR spectroscopy (group task).
Atomic absorption/atomic emission spectroscopy (AAS/AES).
Determination of Zn concentration of zinc tablets using AAS for quality control (group experiment).
Determination of the Ca concentration of mineral waters with AES
Polarimetry
Penumbral polarimeter.
Handling of the penumbral polarimeter.
Investigation of the concentration dependence of the specific rotation.
Photoelectric polarimeter.
Investigation of the influence of different solvents and pH-values on the specific rotation.
Identity testing using the example of the drug N-acetyl-L-cysteine according to the European Pharmacopoeia .
Determination of enantiomeric excess and enantiomeric ratio of the drug (-)-ephedrine hydrochloride .
Specific rotation and optical rotation dispersion of sucrose solution.
ELECTROCHEMISTRY
Potentiometry
Potentiometric precipitation titration: Argentometric titration of potassium bromide
Potentiometric titration of acids and bases:
Amperometry and voltametry
Bivoltametric endpoint determination of redox titrations: Iodometric content determination of sodium thiosulfate.
Coulometry
Classical and modified Karl Fischer method
Water determination in organic solvents.
Polarography - Voltammetry
Theoretical experiments:
Polarograms with an electrolyte solution containing oxygen.
Polarographic determination of copper, lead, cadmium and zinc
Polarographic determination of ascorbic acid in fruit juices
Capillary electrophoresis
Qualitative analysis of drugs by capillary electrophoresis (CE)