Quantitative MRI of organspecific vascular permeability and immune cell dynamics by combination with mass spectrometric imaging

Principal investigators: Cornelius Faber, Uwe Karst
Project number: CRC 1450 B02
Project term: 01/2021–12/2028

Graphical abstract
© CRC inSight

The aim of this project is to develop combined in vivo MRI and ex vivo mass spectrometry imaging (MSI) methods including both, laser ablation-inductively coupled plasma-mass spectrometry imaging (LA-ICP-MSI) and matrix-assisted laser desorption/ionisation mass spectrometry imaging (MALDI-MSI) (1). MSI shall be used to develop fully quantitative MRI methods (2) as part of our comprehensive multiscale imaging strategy (2, 3, 4) to analyse immune responses in disease models and patients in vivo (5).

The overarching question is how quantitative MR parameters depend on elemental and molecular patterns in tissues, and how this dependence evolves during inflammatory responses (6).

A thin tissue section of a mouse brain with a tumour. Left: Image showing autofluorescence using light microscopy. Right: Distribution of an MRI contrast agent based on the metal gadolinium (concentration increased in the tumour region, green to red), acquired through mass spectrometry – this method provides quantitative information about the contrast agent distribution by detecting the mass of gadolinium. We compare the spatial and temporal distribution of the MRI contrast agent with that of a simultaneously applied and structurally similar PET radiotracer (analysed using autoradiography, not shown). Thereby, we aim to validate newly developed mathematical models for processing dynamic PET-MRI imaging data. (Collaboration of projects B02 and B06)
© Katharina Kronenberg/Uwe Karst's research group, samples provided by Florian Gierse/Philipp Backhaus's research team

Team

Principal investigators

Project members

Publications

The names of the principal investigators in our network have been bolded. Publications released prior to 2021, when funding for our network commenced, represent previous project-related work.

2024

Kronenberg K, Hoffmann E, Hiddeßen L, Maus B, Gerwing M, Faber C, Sperling M, Wildgruber M, Karst U. P-based referencing for correcting tissue artifacts in laser ablation-inductively coupled plasma-mass spectrometry imaging of cancer samples. Metallomics 2024;16Abstract
Rivas Aiello MB, Kirse TM, Lavorato GC, Maus B, Maisuls I, Kuberasivakumaran S, Ostendorp S, Hepp A, Holtkamp M, Winkler EL, Karst U, Wilde G, Faber C, Vericat C, Strassert CA. Superparamagnetic Nanoparticles with Phosphorescent Complexes as Hybrid Contrast Agents: Integration of MRI and PLIM. Small Science 2024Abstract
Wilken E, Havlas A, Masthoff M, Moussavi A, Boretius S, Faber C. Radial compressed sensing imaging improves the velocity detection limit of single cell tracking time-lapse MRI. Magn Reson Med 2024;91: 1449-1463. Abstract

2023

Armstrong M, Wilken E, Freppon F, Masthoff M, Faber C, Xiao D. Dynamic cell tracking using time-lapse MRI with variable temporal resolution Cartesian sampling. Magn Reson Med 2023;90: 2443-2453. Abstract
Elinkmann M, Reuter Sa, Holtkamp M, Heuckeroth S, Köhrer A, Kronenberg K, Sperling M, Rubner O, Quarles CD, Hippler M, Karst U. Improving detection thresholds and robust event filtering in single-particle and single-cell ICP-MS analysis. J. Anal. At. Spectrom. 2023Abstract
Heuckeroth S, Behrens A, Wolf C, Fütterer A, Nordhorn ID, Kronenberg K, Brungs C, Korf A, Richter H, Jeibmann A, Karst U, Schmid R. On-tissue dataset-dependent MALDI-TIMS-MS(2) bioimaging. Nat Commun 2023;14: 7495. Abstract
Hoffmann E, Gerwing M, Krähling T, Hansen U, Kronenberg K, Masthoff M, Geyer C, Höltke C, Wachsmuth L, Schinner R, Hoerr V, Heindel W, Karst U, Eisenblätter M, Maus B, Helfen A, Faber C, Wildgruber M. Vascular response patterns to targeted therapies in murine breast cancer models with divergent degrees of malignancy. Breast Cancer Res 2023;25: 56. Abstract
Hoffmann E, Schache D, Höltke C, Soltwisch J, Niland S, Krähling T, Bergander K, Grewer M, Geyer C, Groeneweg L, Eble JA, Vogl T, Roth J, Heindel W, Maus B, Helfen A, Faber C, Wildgruber M, Gerwing M, Hoerr V. Multiparametric chemical exchange saturation transfer MRI detects metabolic changes in breast cancer following immunotherapy. J Transl Med 2023;21: 577. Abstract
Kronenberg K, Werner J, Bohrer P, Steiger K, Buchholz R, von Bremen-Kühne M, Elinkmann M, Paprottka PM, Braren RF, Lohöfer FK, Karst U. Simultaneous quantification of Gadoxetic acid and Cisplatin in hepatocellular carcinomas using laser ablation-inductively coupled plasma-mass spectrometry. Metallomics 2023;15Abstract

2022

Gerwing M, Hoffmann E, Kronenberg K, Hansen U, Masthoff M, Helfen A, Geyer C, Wachsmuth L, Höltke C, Maus B, Hoerr V, Krähling T, Hiddeßen L, Heindel W, Karst U, Kimm MA, Schinner R, Eisenblätter M, Faber C, Wildgruber M. Multiparametric MRI enables for differentiation of different degrees of malignancy in two murine models of breast cancer. Front. Oncol. 2022;12Abstract
Schwarz C, Buchholz R, Jawad M, Hoesker V, Terwesten-Solé C, Karst U, Linsen L, Vogl T, Hoerr V, Wildgruber M, Faber C. Fingerprints of Element Concentrations in Infective Endocarditis Obtained by Mass Spectrometric Imaging and t-Distributed Stochastic Neighbor Embedding. ACS Infect Dis 2022Abstract

2021

Gerwing M, Krähling T, Schliemann C, Harrach S, Schwöppe C, Berdel AF, Klein S, Hartmann W, Wardelmann E, Heindel WL, Lenz G, Berdel WE, Wildgruber M. Multiparametric Magnetic Resonance Imaging for Immediate Target Hit Assessment of CD13-Targeted Tissue Factor tTF-NGR in Advanced Malignant Disease. Cancers (Basel) 2021;13Abstract
Karlas A, Masthoff M, Kallmayer M, Helfen A, Bariotakis M, Fasoula NA, Schäfers M, Seidensticker M, Eckstein H-H, Ntziachristos V, Wildgruber M. Multispectral optoacoustic tomography of peripheral arterial disease based on muscle hemoglobin gradients-a pilot clinical study. Ann Transl Med 2021;9: 36. Abstract
Masthoff M, Freppon FN, Zondler L, Wilken E, Wachsmuth L, Niemann S, Schwarz C, Fredrich I, Havlas A, Block H, Gerwing M, Helfen A, Heindel W, Zarbock A, Wildgruber M, Faber C. Resolving immune cells with patrolling behaviour by magnetic resonance time-lapse single cell tracking. EBioMedicine 2021;73: 103670. Abstract
Spieth L, Berghoff SA, Stumpf SK, Winchenbach J, Michaelis T, Watanabe T, Gerndt N, Düking T, Hofer S, Ruhwedel T, Shaib AH, Willig K, Kronenberg K, Karst U, Frahm J, Rhee JS, Minguet S, Möbius W, Kruse N, von der Brelie C, Michels P, Stadelmann C, Hülper P, Saher G. Anesthesia triggers drug delivery to experimental glioma in mice by hijacking caveolar transport. Neurooncol Adv 2021;3: vdab140. Abstract

2019

Masthoff M, Buchholz R, Beuker A, Wachsmuth L, Kraupner A, Albers F, Freppon F, Helfen A, Gerwing M, Höltke C, Hansen U, Rehkämper J, Vielhaber T, Heindel W, Eisenblätter M, Karst U, Wildgruber M, Faber C. Introducing Specificity to Iron Oxide Nanoparticle Imaging by Combining (57)Fe-Based MRI and Mass Spectrometry. Nano Lett 2019;19: 7908-7917. Abstract

2018

Fingerhut S, Sperling M, Holling M, Niederstadt T, Allkemper T, Radbruch A, Heindel W, Paulus W, Jeibmann A, Karst U. Gadolinium-based contrast agents induce gadolinium deposits in cerebral vessel walls, while the neuropil is not affected: an autopsy study. Acta Neuropathol 2018;136: 127-138. Abstract
Kröger S, Niehoff A-C, Jeibmann A, Sperling M, Paulus W, Stummer W, Karst U. Complementary Molecular and Elemental Mass-Spectrometric Imaging of Human Brain Tumors Resected by Fluorescence-Guided Surgery. Anal Chem 2018;90: 12253-12260. Abstract
Lohöfer F, Hoffmann L, Buchholz R, Huber K, Glinzer A, Kosanke K, Feuchtinger A, Aichler M, Feuerecker B, Kaissis G, Rummeny EJ, Höltke C, Faber C, Schilling F, Botnar RM, Walch AK, Karst U, Wildgruber M. Molecular imaging of myocardial infarction with Gadofluorine P - A combined magnetic resonance and mass spectrometry imaging approach. Heliyon 2018;4: e00606. Abstract
Masthoff M, Gran S, Zhang X, Wachsmuth L, Bietenbeck M, Helfen A, Heindel W, Sorokin L, Roth J, Eisenblätter M, Wildgruber M, Faber C. Temporal window for detection of inflammatory disease using dynamic cell tracking with time-lapse MRI. Sci Rep 2018;8: 9563. Abstract

2017

Niehoff A-C, Klasen T, Schmidt R, Palmes D, Faber C, Karst U, Hadrian R. Investigating the Lymphatic System by Dual-Color Elemental Mass Spectrometry Imaging. Contrast Media Mol Imaging 2017;2017: 4035721. Abstract