FL module

 

The FL-module is based upon a synthetically designed, 82aa long peptide that adopts a ferrodoxin (FL)-like fold in the absence of mechanical tension. Loading the mechanosensitive peptide with 3–5 pN leads to a quick transition into the unfolded state resulting in a FRET loss. In comparison with the F40-module, the FL-module benefits from a higher FRET efficiency at zero force, and enlarged contour length increase under tension resulting in an improved dynamic range. In addition, the FL-module is rather insensitive to forces of about 1–2 pN and therefore less prone to mechanical noise. The force response is virtually digital, which can be harnessed to estimate the fraction of mechanically engaged molecules from FLIM-based ensemble measurements (Ringer et al. 2017).

© Uni MS - Prof. Dr. C. Grashoff

 

Ringer P, Weißl A, Cost AL, Freikamp A, Sabass B, Mehlich A, Tramier M, Rief M, Grashoff C. Nature Methods 2017 Nov; 14(11):1090-1096. [Epub 2017 Sep 18] >>