Advanced studies (Master of Science, MSc):
Research Module
Scheduled by arrangement; Practical course; daily, 8 weeks (~6 weeks in the lab and ~2 weeks for preparation of the protocol and an oral presentation)
The content is adapted continuously to current research topics within the working group. Frequently used standard techniques are molecular cloning of recombinant DNA-constructs, expression analysis by qRT-PCR, recombinant protein expression in bacteria, yeast, and plants, confocal laser scanning microscopy (e.g. (multicolour)BIFC, FLIM-FRET), protein biochemistry, enzyme assays, extraction of plant secondary metabolites, GC/MS-analysis, HPLC.
If you are interested in joining our group for a Research Module, please contact us!
gnoll@uni-muenster.de or nicole.vandeenen@uni-muenster.de
Advanced Modules:
Molekulare Biotechnologie der Pflanzen (Molecular Plant Biotechnology)
SuSe or WiSe; Practical course with accompanying theory sessions; daily, 4 weeks
The course content is adapted annually to current research topics within the working group. Frequently used standard techniques are molecular cloning of recombinant DNA-constructs, expression analysis by RT-PCR, recombinant protein expression in bacteria, yeast, and plants, confocal laser scanning microscopy, protein biochemistry & enzyme assays.
Genome Editing in Plants
SuSe or WiSe; Practical course with accompanying theory sessions; daily, 4 weeks
In this course, we teach the basics for successful genome editing in plants and potential pitfalls are highlighted. The acquired knowledge is directly applied by selecting a suitable target sequence, designing and molecularly cloning protospacers, performing transient plant transformations, and finally screening for CRISPR-edits in the genomes of plants using three different methods. In addition, the progeny of CRISPR-edited allotetraploid tobacco plants are screened for zygosity, highlighting the importance of understanding Mendelian inheritance in diploid and polyploid plants to efficiently develop individual experimental designs. Thus, this course will provide you with the key knowledge and tools that should enable you to independently design and conduct successful genome editing experiments in the future.
Molekulare Phytopathologie (Molecular Phytopathology)
SuSe; Joint practical course held by AG Prüfer, AG Moerschbacher and AG Schwarzländer, with accompanying theory sessions; daily, 4 weeks
This course covers various topics in phytopathology as students spend a week in each of the different AGs working on their respective issues. The AG Prüfer week deals with the first virus ever described, the Tobacco Mosaic Virus (TMV). Here, the focus is on compatible and non-compatible plant-virus interactions. Additionally, the participants will use plant viruses as biotechnological platforms for the bulk production of proteins.
Herbstschule »Molekulare Pflanzenzüchtung für eine nachhaltige Entwicklung« (Molecular plant breeding for sustainable development)
Scheduled by arrangement; Lectures, seminars, practical work, excursions (Münster, Carolinensiel)
The Fall School is a joint project of the Universities of Lüneburg, Münster, Hannover and Rostock and celebrated its 10th anniversary in 2019. It is an interdisciplinary event for students of biological, agricultural, horticultural, and business studies that covers the fundamentals of sustainable agricultural systems (conventional, organic, and genetic) as well as social aspects of the genetic engineering debate. Part of the event will take place at the Marine Biological Station of the WWU Münster in Carolinensiel. Topic complexes of the school include questions on classical and modern plant breeding (genetic engineering, genome editing CRISPR & Co., etc.), sustainability of different cultivation systems, bioethics, communication, public acceptance and stakeholder management.
Master thesis
Approx. 9 months, daily
Individual project linked to current research topics, supervised by a PhD student and/or a Post-Doc.
The content is adapted continuously to current research topics within the working group. Frequently used standard techniques are molecular cloning of recombinant DNA-constructs, expression analysis by qRT-PCR, recombinant protein expression in bacteria, yeast, and plants, confocal laser scanning microscopy (e.g. (multicolour)BIFC, FLIM-FRET), protein biochemistry, enzyme assays, extraction of plant secondary metabolites, GC/MS-analysis, HPLC.
For further information, please also check the online Modulhandbuch!