Prof. Dr. Jürgen Klingauf

Prof. Dr. Jürgen Klingauf

Busso-Peus-Straße 10
48149 Münster

T: +49 251 83-51001

  • Forschungsschwerpunkte

    • Mechanisms of exo- and endocyosis, with particular emphasis on synaptic vesicle recycling
    • cell physiology of synaptic transmission using electrophysiology and high-resolution imaging techniques

  • Weitere Zugehörigkeit an der Universität Münster

  • Vita

    Akademische Ausbildung

    PhD in Physics (Dr. rer. nat.), University of Göttingen
    Degree Student with Prof. Erwin Neher, Max Planck Institute for Biophysical Chemistry, Göttingen
    Studies of Biology and Physics, Universities of Hamburg and Bonn

    Beruflicher Werdegang

    Professor (W3) of Medical Physics and Biophysics, Medical Faculty, University of Münster (WWU)
    Head of Institute of Medical Physics and Biophysics, University of Münster (WWU)
    Independent Research Group Leader, MPI Biophysical Chemistry, Göttingen
    Postdoc with Prof. Erwin Neher, Department of Membrane Biophysics, MPI Biophysical Chemistry, Göttingen
    Guest Researcher, Department of Molecular Cellular Physiology, Stanford Medical School, California, USA

    Preise

    PhD-Stipendium – Boehringer Ingelheim Fonds (BIF)
    Grant from the Human Frontier Science Program Organisation – International Human Frontier Science Program Organization (HFSP)

    Mitgliedschaften und Aktivitäten in Gremien

    Member of the Neuroscience Graduate Study Programme, Max Planck Institute and the University, Göttingen
    Member of the Society for Neuroscience, Washington D.C., USA
    Member of the American Biophysical Society, Bethesda, MD, USA
    Member of the German Society of Physiology, Rostock

    Rufe

    Offer, Professorship, Cluster of Excellence Macromolecular Complexes, University of Frankfurt (declined)
    , Cluster of Excellence Macromolecular Complexes – abgelehnt
    Offer, Professorship at the Medical School, Yale University, New Haven, CT, USA (declined)
    – abgelehnt

  • Projekte

    • SFB 1348: Dynamische zelluläre Grenzflächen - Bildung und Funktion ( – )
      DFG-Hauptprojekt koordiniert an der Universität Münster: DFG - Sonderforschungsbereich | Förderkennzeichen: SFB 1348/2
    • SFB 1348 A02 - Lokale Regulation der engen Kopplung von synaptischer Exo- und Endocytose ( – )
      Teilprojekt in DFG-Verbund koordiniert an der Universität Münster: DFG - Sonderforschungsbereich | Förderkennzeichen: SFB 1348/2 A02
    • SFB 1348 - Dynamische zelluläre Grenzflächen: Bildung und Funktion ( – )
      DFG-Hauptprojekt koordiniert an der Universität Münster: DFG - Sonderforschungsbereich | Förderkennzeichen: SFB 1348/1
    • SFB 1348 A02 - Lokale Regulation der engen Kopplung von synaptischer Exo- und Endocytose ( – )
      Teilprojekt in DFG-Verbund koordiniert an der Universität Münster: DFG - Sonderforschungsbereich | Förderkennzeichen: SFB 1348/1 A02
    • EVO-CELL – KMU-innovativ-21: EVO-CELL - Entwicklung einer multiparametrischen Zellanalysetechnologie für die Erforschung und Entwicklung zellbasierter Therapien ( – )
      participations in bmbf-joint project: Bundesministerium für Bildung und Forschung | Förderkennzeichen: 031B0654B
    • EXC 1003 A3 - Funktionelle Membrandomänen bei neuronaler und nicht-neuronaler Kompartimentierung ( – )
      Teilprojekt in DFG-Verbund koordiniert an der Universität Münster: DFG - Exzellenzcluster | Förderkennzeichen: EXC1003/1
    • EXC 1003 FF-2017-10 - Biohybrid neurosynaptic chips interfaced with nanostructured, integrated optics ( – )
      Teilprojekt in DFG-Verbund koordiniert an der Universität Münster: DFG - Exzellenzcluster | Förderkennzeichen: FF-2017-10
    • EXC 1003 FF-2015-05 – Direct neuronal Interfacing on functionalized microstructured coverslips for imaging synapse development and plasticity ( – )
      Teilprojekt in DFG-Verbund koordiniert an der Universität Münster: DFG - Exzellenzcluster
    • SFB 629 A17 - Lipid- und Protein-Sortierung und Recyclierung während der synaptischen Übertragung ( – )
      Teilprojekt in DFG-Verbund koordiniert an der Universität Münster: DFG - Sonderforschungsbereich | Förderkennzeichen: SFB629A017
    • VesDyn – Synaptic vesicle acidification and refilling dynamics ( – )
      EU-Projekt koordiniert an der Universität Münster: EU FP 7 - Marie Curie Actions - Innereuropäische Stipendien
    • CiM-IMPRS – Cells in Motion (CiM) Graduate School and International Max Planck Research School - Molecular Biomedicine (seit )
      Gefördertes Einzelprojekt: Ministerium für Kultur und Wissenschaft des Landes Nordrhein-Westfalen
    • EuroSynapse - Spatio-temporal organization of the synaptic membrane for synaptic vesicle protein recycling ( – )
      Gefördertes Einzelprojekt: DFG - Sachbeihilfe/Einzelförderung | Förderkennzeichen: KL 1334/2-1
    • Schaltbare Nanopartikel als Wirkstoffträger ( – )
      Eigenmittelprojekt
    • Sichtbarmachung der Dynamik von synaptischen Vesikelproteinen nach der Fusion ( – )
      Gefördertes Einzelprojekt: DFG - Sachbeihilfe/Einzelförderung | Förderkennzeichen: KL 1334/1-1

  • Publikationen

    • Singh A; Thale S; Leibner T; Lamparter L; Ricker A; Nüsse H; Klingauf J; Galic M; Ohlberger M; Matis M. . ‘Dynamic interplay of microtubule and actomyosin forces drive tissue extension.’ Nature Communications 15, Nr. 1: 3198. doi: 10.1038/s41467-024-47596-8.
    • Schnieder, M.; Niemann, A.; Hüve, J.; Klingauf, J. . ‘Collimating three-axicon zoom system for interferometric Bessel beam side lobe cancellation.’ Optical and Quantum Electronics 56: 716. doi: 10.1007/s11082-023-06229-y.
    • Schnieder, M.; Voß, F.; Hüve, J.; Klingauf, J. „Combining Dual-View Plane Illumination Microscopy with Bessel Beams.“ contributed to the Focus on Microscopy, Porto, .
    • Schnieder M., Niemann A., Hüve J., Klingauf J. „Power Efficient 3-Axicon System for Generation of Tuneable Bessel Beams.“ contributed to the Focus on Microscopy, Online, .
    • Goossen-Schmidt N.C., Schnieder M., Hüve J., Klingauf J. . ‘Switching behaviour of dSTORM dyes in glycerol-containing buffer.’ Scientific Reports 10. doi: 10.1038/s41598-020-70335-0.
    • Kaspar C, Lehrich J, Ivanenko A, Klingauf J, Pernie W. . ‘Integrated photonics chip for neural activity investigation.’ Optogenetics and Optical Manipulation 11227. doi: 10.1117/12.2546183.
    • Bergemann I, Saha T, Lamparter L, Rathmann I, Grill D, Golbach L, Rasch C, Keller U. Trappmann B, Matis M, Gerke V, Klingauf J, Galic M. . ‘Mechanochemical self-organization determines search pattern in migratory cells.’ Nature Physics 15. doi: 10.1038/s41567-019-0505-9.
    • Schmidt N.C., Schnieder M., Hüve J., Klingauf J. „Intrinsic Refractive Index Matched 3D dSTORM with Two Objectives.“ contributed to the Focus On Microscopy, London, Vereinigtes Königreich, .
    • Dlugos, C. P.; Picciotto, C.; Lepa, C.; Krakow, M.; Stober, A.; Eddy, M. L.; Weide, T.; Jeibmann, A.; Krahn M. P. ; Van Marck, V.; Klingauf, J. Ricker, A. Wedlich-Soldner, R. Pavenstadt, H. Klambt, C. George, B. . ‘Nephrin Signaling Results in Integrin beta1 Activation.’ Journal of the American Society of Nephrology 30, Nr. 6: 1006–1019. doi: 10.1681/ASN.2018040362.
    • Schmidt Nora C., Kahms Martin, Hüve Jana , Klingauf Jürgen. . ‘Intrinsic refractive index matched 3D dSTORM with two objectives: Comparison of detection techniques.’ Scientific Reports 8. doi: 10.1038/s41598-018-31595-z.
    • Schmidt N C, Hüve J , Klingauf J. „3D Pointillism Microscopy setup with two objectives.“ contributed to the DPG-Frühjahrstagung, Hannover, Deutschland, .
    • Rajappa R., Gauthier-Kemper A., Böning D., Hüve J., Klingauf J. . ‘Synaptophysin 1 Clears Synaptobrevin 2 from the Presynaptic Active Zone to Prevent Short-Term Depression.’ Cell Reports 14, Nr. 6: 1369–1381. doi: 10.1016/j.celrep.2016.01.031.
    • Schmidt N C, Hüve J, Klingauf J. „Dual-Objective Pointillism Microscopy Setup with Interferometric and Astigmatic Detection.“ contributed to the Biophysical Society 59th Annual Meeting, Baltimore, Maryland, USA, .
    • Gauthier-Kemper A, Rajappa R, Wiemhöfer M, Thiel CS, Hüve J, Klingauf J. „Syp1 Acts as a Clearance Factor for Syb2 at the Presynapse.“ contributed to the Biophysical Society 58th annual meeting, San Francisco, .
    • Trahe J, Hüve J, Selenschik P, Glyvuk N, Gauthier-Kemper A, Piehler J, Klingauf J. . ‘Guided Growth of Neurons on Micro-Structured Surfaces.’ Contributed to the Biophysical Society 58th annual meeting, San Francisco.
    • Sinha R, Ahmed S, Jahn R, Klingauf J. . ‘Two synaptobrevin molecules are sufficient for vesicle fusion in central nervous system synapses.’ Proceedings of the National Academy of Sciences of the United States of America 108, Nr. 34: 14318–14323. doi: 10.1073/pnas.1101818108.
    • Sinha R, Ahmed S, Jahn R, Klingauf J. . ‘Two synaptobrevin molecules are sufficient for vesicle fusion in central nervous system synapses.’ Proceedings of the National Academy of Sciences of the United States of America 108, Nr. 34: 14318–14323.
    • Groemer TW, Thiel CS, Holt M, Riedel D, Hua Y, Hüve J, Wilhelm BG, Klingauf J. . ‘Amyloid precursor protein is trafficked and secreted via synaptic vesicles.’ PloS one 6, Nr. 4: e18754.
    • Hua Y, Sinha R, Thiel CS, Schmidt R, Hüve J, Martens H, Hell SW, Egner A, Klingauf J. . ‘A readily retrievable pool of synaptic vesicles.’ Nature Neuroscience 14, Nr. 7: 833–835.
    • Mothet JP, Shi T, Puyal J, Klingauf J, Sweedler J, Jahn R, Martineau M. . ‘VESICULAR TRANSPORT OF D-SERINE IN ASTROCYTES.’ Glia 59 1: S34–S35.
    • Hua YF, Sinha R, Thiel CS, Schmidt R, Huve J, Martens H, Hell SW, Egner A, Klingauf J. . ‘A readily retrievable pool of synaptic vesicles.’ Nature Neuroscience 14, Nr. 7: 833. doi: 10.1038/nn.2838.
    • Glyvuk N, Tsytsyura Y, Geumann C, D'Hooge R, Huve J, Kratzke M, Baltes J, Boening D, Klingauf J, Schu P. . ‘AP-1/sigma 1B-adaptin mediates endosomal synaptic vesicle recycling, learning and memory (vol 29, pg 1318, 2010).’ EMBO Journal 29, Nr. 11: 1941–1941.
    • Boening D, Groemer TW, Klingauf J. . ‘Applicability of an EM-CCD for spatially resolved TIR-ICS.’ Plant Methods 18, Nr. 13: 13516–13528. doi: 10.1364/OE.18.013516.
    • Hua Y, Sinha R, Martineau M, Kahms M, Klingauf J. . ‘A common origin of synaptic vesicles undergoing evoked and spontaneous fusion.’ Nature Neuroscience 13, Nr. 12: 1451–1453. doi: 10.1038/nn.2695.
    • Glyvuk N, Tsytsyura Y, Geumann C, D'Hooge R, Hüve J, Kratzke M, Baltes J, Boening D, Böning D, Klingauf J, Schu P. . ‘AP-1/sigma1B-adaptin mediates endosomal synaptic vesicle recycling, learning and memory.’ EMBO Journal 29, Nr. 8: 1318–1330. doi: 10.1038/emboj.2010.15.
    • Boening D, Groemer TW, Klingauf J. . ‘Applicability of an EM-CCD for spatially resolved TIR-ICS.’ Optics Express 18, Nr. 13: 13516–13528. doi: 10.1364/OE.18.013516.
    • Glyvuk N, Tsytsyura Y, Geumann C, D'Hooge R, Huve J, Kratzke M, Baltes J, Boening D, Klingauf J, Schu P. . ‘AP-1/sigma 1B-adaptin mediates endosomal synaptic vesicle recycling, learning and memory .’ EMBO Journal 29, Nr. 11: 1941. doi: 10.1038/emboj.2010.96.
    • Glyvuk N, Tsytsyura Y, Geumann C, D'Hooge R, Huve J, Kratzke M, Baltes J, Boning D, Klingauf J, Schu P. . ‘AP-1/sigma 1B-adaptin mediates endosomal synaptic vesicle recycling, learning and memory.’ EMBO Journal 29, Nr. 8: 1318–1330. doi: 10.1038/emboj.2010.15.
    • Welzel O, Boening D, Stroebel A, Reulbach U, Klingauf J, Kornhuber J, Groemer TW. . ‘Determination of axonal transport velocities via image cross- and autocorrelation.’ European Biophysics Journal 38, Nr. 7: 883–889. doi: 10.1007/s00249-009-0458-5.
    • Medigeshi GR, Krikunova M, Radhakrishnan K, Wenzel D, Klingauf J, Schu P. . ‘AP-1 membrane-cytoplasm recycling regulated by mu 1A-adaptin.’ Traffic 9, Nr. 1: 121–132. doi: 10.1111/j.1600-0854.2007.00672.x.
    • Jung N, Wienisch M, Gu M, Rand JB, Muller SL, Krause G, Jorgensen EM, Klingauf J, Haucke V. . ‘Molecular basis of synaptic vesicle cargo recognition by the endocytic sorting adaptor stonin 2.’ Journal of Cell Biology 179, Nr. 7: 1497–1510. doi: 10.1083/jcb.200708107.
    • Gulyas-Kovacs A, de Wit H, Milosevic I, Kochubey O, Toonen R, Klingauf J, Verhage M, Sorensen JB. . ‘Munc18-1: Sequential interactions with the fusion machinery stimulate vesicle docking and priming.’ Journal of Neuroscience 27, Nr. 32: 8676–8686.
    • Tsytsyura Y, Glyvuk N, Riel C, Baltes J, Schu P, Klingauf J. . ‘A role for the endosomal adaptor protein AP1 in synaptic vesicle cycling.’ Journal of General Physiology 130, Nr. 1: 15a16a.
    • Thiel CS, Groemer TW, Wienands J, Klingauf J. . ‘A role for the F-actin binding protein mAbp1 in clathrin-mediated endocytosis.’ Journal of General Physiology 130, Nr. 1: 15a15a.
    • Klingauf J. . ‘Coupling of exo- and endocytosis at CNS synapses.’ Journal of General Physiology 130, Nr. 1: 4a4a.
    • Henkel AW, Dittrich PS, Groemer TW, Lemke EA, Klingauf J, Klafki HW, Lewczuk P, Esselmann H, Schwille P, Kornhuber J, Wiltfang J. . ‘Immune complexes of auto-antibodies against A beta 1-42 peptides patrol cerebrospinal fluid of non-Alzheimer's patients.’ Molecular Psychiatry 12, Nr. 6: 601–610. doi: 10.1038/sj.mp.4001947.
    • Klingauf J. . ‘Synaptic vesicle dynamics sans dynamin.’ Neuron 54, Nr. 6: 857–858.
    • Vanden Berghe P, Klingauf J. . ‘Spatial organization and dynamic properties of neurotransmitter release sites in the enteric nervous system.’ Neuroscience 145, Nr. 1: 88–99.
    • Groemer TW, Klingauf J. . ‘Synaptic vesicles recycling spontaneously and during activity belong to the same vesicle pool.’ Nature Neuroscience 10, Nr. 2: 145–147. doi: 10.1038/nn1831.
    • Kochubey O, Majumdar A, Klingauf J. . ‘Imaging clathrin dynamics in Drosophila melanogaster hemocytes reveals a role for actin in vesicle fission.’ Traffic 7, Nr. 12: 1614–1627. doi: 10.1111/j.1600-0854.2006.00492.x.
    • Takamori S, Holt M, Stenius K, Lemke EA, Gronborg M, Riedel D, Urlaub H, Schenck S, Brugger B, Ringler P, Muller SA, Rammner B, Grater F, Hub JS, De Groot BL, Mieskes G, Moriyama Y, Klingauf J, Grubmuller H, Heuser J, Wieland F, Jahn R. . ‘Molecular anatomy of a trafficking organelle.’ Cell 127, Nr. 4: 831–846. doi: 10.1016/j.cell.2006.10.030.
    • Wienisch M, Klingauf J. . ‘Vesicular proteins exocytosed and subsequently retrieved by compensatory endocytosis are nonidentical.’ Nature Neuroscience 9, Nr. 8: 1019–1027. doi: 10.1038/nn1739.
    • Toonen RF, Kochubey O, de Wit H, Gulyas-Kovacs A, Konijnenburg B, Sorensen JB, Klingauf J, Verhage M. . ‘Dissecting docking and tethering of secretory vesicles at the target membrane.’ EMBO Journal 25, Nr. 16: 3725–3737. doi: 10.1038/sj.emboj.7601256.
    • Vanden Berghe P, Klingauf J. . ‘Synaptic vesicles in rat hippocampal boutons recycle to different pools in a use-dependent fashion.’ Journal of Physiology 572, Nr. 3: 707–720. doi: 10.1113/jphysiol.2005.100842.
    • Connert S, Wienand S, Thiel C, Krikunova M, Glyvuk N, Tsytsyura Y, Hilfiker-Kleiner D, Bartsch JW, Klingauf J, Wienands J. . ‘SH3P7/mAbp1 deficiency leads to tissue and behavioral abnormalities and impaired vesicle transport.’ EMBO Journal 25, Nr. 8: 1611–1622.
    • Diril MK, Wienisch M, Jung N, Klingauf J, Haucke V. . ‘Stonin 2 is an AP-2-dependent endocytic sorting adaptor for synaptotagmin internalization and recycling.’ Developmental Cell 10, Nr. 2: 233–244. doi: 10.1016/j.devcel.2005.12.011.
    • Lemke EA, Klingauf J. . ‘Single synaptic vesicle tracking in individual hippocampal boutons at rest and during synaptic activity.’ Journal of Neuroscience 25, Nr. 47: 11034–11044.
    • Loerke D, Wienisch M, Kochubey O, Klingauf J. . ‘Differential control of clathrin subunit dynamics measured with EW-FRAP microscopy.’ Traffic 6, Nr. 10: 918–929. doi: 10.1111/j.1600-0854.2005.00329.x.
    • Jordan R, Lemke EA, Klingauf J. . ‘Visualization of synaptic vesicle movement in intact synaptic boutons using fluorescence fluctuation spectroscopy.’ Biophysical Journal 89, Nr. 3: 2091–2102. doi: 10.1529/biophysj.105.061663.
    • Mueller VJ, Wienisch M, Nehring RB, Klingauf J. . ‘Monitoring clathrin-mediated endocytosis during synaptic activity.’ Journal of Neuroscience 24, Nr. 8: 2004–2012. doi: 10.1523/JNEUROSCI.4080-03.2004.
    • Choi S, Klingauf J, Tsien RW. . ‘Fusion pore modulation as a presynaptic mechanism contributing to expression of long-term potentiation.’ Philosophical Transactions of the Royal Society B: Biological Sciences 358, Nr. 1432: 695–705. doi: 10.1098/rstb.2002.1249.
    • Lemke EA, Klingauf J. . ‘Visualization of single synaptic vesicle dynamics in hippocampal boutons by two-photon laser-scanning microscopy.’ Biophysical Journal 84, Nr. 2: 211A211A.
    • Wienisch M, Klingauf J. . ‘A slow endocytosis pathway in hippocampal synapses.’ Biophysical Journal 82, Nr. 1: 616A617A.
    • Bundy A, Loerke D, Klingauf J. . ‘A synaptosomal preparation for studying vesicle cycling by evanescent wave microscopy.’ Biophysical Journal 82, Nr. 1: 615A.
    • Wienisch M, Muller VJ, Nehring RB, Klingauf J. . ‘Monitoring clathrin-mediated endocytosis in hippocampal synapses.’ Biophysical Journal 82, Nr. 1: 10A10A.
    • Jordan R, Klingauf J. . ‘Vesicle dynamics in hippocampal boutons visualised by fluorescence spectroscopy.’ European Journal of Neuroscience 12: 367.
    • Bruns D, Riedel D, Klingauf J, Jahn R. . ‘Quantal release of serotonin.’ Neuron 28, Nr. 1: 205–220.
    • Choi S, Klingauf J, Tsien RW. . ‘Postfusional regulation of cleft glutamate concentration during LTP at 'silent synapses'.’ Nature Neuroscience 3, Nr. 4: 330–336.
    • Jordan R, Klingauf J. . ‘Vesicle dynamics in hippocampal boutons in culture visualized by fluorescence correlation spectroscopy.’ Biophysical Journal 78, Nr. 1: 260A260A.
    • Klingauf J, Kavalali ET, Tsien RW. . ‘Kinetics and regulation of fast endocytosis at hippocampal synapses.’ Nature 394, Nr. 6693: 581–585. doi: 10.1038/29079.

  • Betreute Promotion

    The Role of Giant Ankyrins in the Organization and Stabilization of the Synaptic Cytoskeleton at the Drosophila Neuromuscular Junction