Publikationen
- Structure of reverse gyrase with a minimal latch that supports ATP-dependent positive supercoiling without specific interactions with the topoisomerase domain . In: Acta Crystallographica Section D: Structural Biology 79 (), №. - 6. doi: 10.1107/S2059798323002565.
- Linking translation factor conformational dynamics with translation efficiencies and gene expression . In: Biophysical Journal 121 (), №. - 3, pp. 338a–339a. doi: .
- Analysis of the conformational space and dynamics of RNA helicases by single-molecule FRET in solution and on surfaces . In: Methods in Enzymology 673 (), pp. 251–310. doi: 10.1016/bs.mie.2022.03.043.
- The domains of yeast eIF4G, eIF4E and the cap fine-tune eIF4A activities through an intricate network of stimulatory and inhibitory effects . In: Nucleic Acids Research 50 (), №. - 11, pp. 6497–6510. doi: 10.1093/nar/gkac437.
- Determination of rate constants for conformational changes of RNA helicases by single-molecule FRET TIRF microscopy . In: Methods 204 (), pp. 428–441. doi: 10.1016/j.ymeth.2022.03.004.
- What makes a type IIA topoisomerase a gyrase or a Topo IV? . In: Nucleic Acids Res . 2021 (). doi: 10.1093/nar/gkab270.
- Towards Conformation-Sensitive Inhibition of Gyrase: Implications of Mechanistic Insight for the Identification and Improvement of Inhibitors . In: Molecules . 26 (), №. - 5. doi: 10.3390/molecules26051234..
- Regulation of RNA helicase activity: principles and examples . In: Biol Chem . 2021 (). doi: 10.1515/hsz-2020-0362.
- Probing RNA Helicase Conformational Changes by Single-Molecule FRET Microscopy . In: Methods Mol Biol 2209 (), pp. 119–132. doi: 10.1007/978-1-0716-0935-4_8.
- Highlight: RNA helicases–structure, function, mechanism and regulation . In: Biological Chemistry Hoppe-Seyler 402 (), №. - 5, pp. 527–528. doi: 10.1515/hsz-2021-0194.
- Conjugates of ciprofloxacin and amphiphilic block copoly (2-alkyl-2-oxazolines) s overcome efflux pumps and are active against CIP-resistant bacteria . In: Molecular pharmaceutics 18 (), №. - 9, pp. 3532–3543. doi: 10.1021/acs.molpharmaceut.1c00430.
- The Thermus thermophilus DEAD-box protein Hera is a general RNA binding protein and plays a key role in tRNA metabolism . In: RNA . 11 (), pp. 1557–1574. doi: 10.1261/rna.075580.120.
- A β-hairpin is a Minimal Latch that Supports Positive Supercoiling by Reverse Gyrase . In: J Mol Biol 432 (), №. - 16, pp. 4762–4771. doi: 10.1016/j.jmb.2020.06.018.
- Allosteric modulation of the GTPase activity of a bacterial LRRK2 homolog by conformation-specific Nanobodies . In: Biochem J . 477 (), №. - 7, pp. 1203–1218. doi: 10.1042/BCJ20190843.
- Functional interactions between gyrase subunits are optimized in a species-specific manner . In: J Biol Chem . 295 (), №. - 8, pp. 2299–2312. doi: 10.1074/jbc.RA119.010245.
- Single-stranded regions modulate conformational dynamics and ATPase activity of eIF4A to optimize 5'-UTR unwinding . In: Nucleic Acids Res . 47 (), №. - 10, pp. 5260–5275. doi: 10.1093/nar/gkz254.
- Gyrase containing a single C-terminal domain catalyzes negative supercoiling of DNA by decreasing the linking number in steps of two . In: Nucleic Acids Research 46 (), №. - 13, pp. 6773–6784. doi: 10.1093/nar/gky470.
- Why Two? On the Role of (A-)Symmetry in Negative Supercoiling of DNA by Gyrase . In: Int. J. Mol. Sci. 19 (). doi: 10.3390/ijms19051489.
- Binding and hydrolysis of a single ATP is sufficient for negative supercoiling of DNA by gyrase . In: J. Mol. Biol. 429 (), №. - 23, pp. 3717–3729. doi: 10.1016/j.jmb.2017.10.005.
- Allosteric regulation of helicase core activities of the DEAD-box helicase YxiN by RNA binding to its RNA recognitation motif . In: Nuc. Acids Res. 45 (), №. - 4, pp. 1994–2006. doi: 10.1093/nar/gkx014.
- Biophysical Chemistry. N/A: unbekannt / n.a. / unknown. , . ISBN 9781482252231. doi: .
- eIF4B stimulates eIF4A ATPase and unwinding activities by direct interaction through its 7-repeats region . In: RNA Biol. 14 (), №. - 1, pp. 113–123. doi: 10.1080/15476286.2016.1259782.
- Single-Molecule Confocal FRET Microscopy to Dissect Conformational Changes in the Catalytic Cycle of DNA Topoisomerases . In: Methods Enzymol 581 (), pp. 317–351. doi: 10.1016/bs.mie.2016.08.013.
- DNA gyrase with a single catalytic tyrosine can catalyze DNA supercoiling by a nicking-closing mechanism . In: Nucl. Acids Res. 44 (), №. - 21, pp. 10354–10366. doi: 10.1093/nar/gkw740.
- Protein cofactor competition regulates the action of a multifunctional RNA helicase in different pathways . In: RNA Biol 13 (), №. - 3, pp. 320–330. doi: 10.1080/15476286.2016.1142038.
- When core competence is not enough: functional interplay of the DEAD-box helicase core with ancillary domains and auxilliary factors in RNA binding and unwinding . In: Biol. Chem. 396 (), №. - 8, pp. 849–865. doi: 10.1515/hsz-2014-0277.
- Nickel quercetinase, a "promiscuous metalloenzyme: metal incorporation and metal ligand substitution studies . In: BMC Biochem 16 (), №. - 10. doi: 10.1186/s12858-015-0039-4.
- DEAD-box RNA helicase domains exhibit a continuum between complete functional independence and high thermodynamic coupling in nucleotide and RNA duplex recognition . In: Nucl. Acids Res 42 (), №. - 16, pp. 10644–10654. doi: .
- Differential contributions of the latch in Thermotoga maritima reverse gyrase to binding of single-stranded DNA before and after ATP hydrolysis . In: Biol. Chem. 395 (), №. - 1, pp. 83–93. doi: 10.1515/hsz-2013-0177.
- eIF4B and eIF4G jointly stimulate eIF4A ATPase and unwinding activities by modulation of the eIF4A conformational cycle . In: J. Mol. Biol. 426 (), №. - 1, pp. 51–61. doi: 10.1016/j.jmb.2013.09.027.
- The acidic C-terminal tail of the GyrA subunit moderates the DNA supercoiling activity of Bacillus subtilis gyrase . In: J. Biol. Chem. 289 (), №. - 18, pp. 12275–12285. doi: 10.1074/jbc.M114.547745.
- The mechanism of negative DNA supercoiling: a cascade of DNA-induced conformational changes prepares gyrase for strand passage . In: DNA Repair 16 (), pp. 23–24. doi: 10.1016/j.dnarep.2014.01.011.
- eIF4B, eIF4G and RNA regulate eIF4A activity in translation initiation by modulating the eIF4A conformational cycle . In: Nucl. Acids Res. 42 (), №. - 12, pp. 7911–7922. doi: 10.1093/nar/gku440.
- Reverse gyrase-recent advances and current mechanistic understanding of positive DNA supercoiling . In: Nucl. Acids Res. 42 (), pp. 8200–8213. doi: 10.1093/nar/gku589.
- Fluorescence Methods in the Investigation of the DEAD-Box Helicase Mechanism . In: Experienta Supplementum 105 (), pp. 161–192. doi: 10.1007/978-3-0348-0856-9_8.
- Rearranging RNA structures at 75°C? Towards the physiologic function of the DEAD-box helicase Hera from Thermus thermophilus”, for the Biopolymers Special Issue “Nucleic acids . In: Biopolymers 99 (), №. - 12, pp. 1137–1146. doi: 10.1002/bip.22316.
- Single molecule FRET reveals DNA- and nucleotide-induced conformational changes in DNA gyrase preceding the strand passage reaction, for the DNA repair Special Issue “Single molecule approaches: watching DNA repair one molecule at a time . In: DNA repair (). doi: 10.1042/BST0390611.
- Differential contributions of the latch in Thermotoga maritima reverse gyrase to binding of single-stranded DNA before and after ATP hydrolysis . In: Biol. Chem. 394 (). doi: 10.1515/hsz-2013-0177. [online first]
- eIF4B and eIF4G jointly stimulate eIF4A ATPase and unwinding activities by modulation of the eIF4A conformational cycle . In: J. Mol. Biol. 2013 (). doi: 10.1016/j.jmb.2013.09.027.. [online first]
- Recognition of two distinct elements in the RNA substrate by the RNA-binding domain of the T. thermophilus DEAD box helicase Hera . In: Nucl. Acids Res. 41 (), №. - 12, pp. 6259–6272. doi: 10.1093/nar/gkt323.
- Mapping the Spectrum of Conformational States of the DNA- and C-Gates in Bacillus subtilis Gyrase . In: J. Mol. Biol. 2013 (). doi: .
- Lifelong companions: RNA helicases and their roles in RNA metabolism . In: RNA Biol. 10 (), №. - 1, pp. 2–3. doi: .
- Crystal structures of Thermotoga maritima reverse gyrase: inferences for the mechanism of positive DNA supercoiling . In: Nucl. Acids Res. 41 (), №. - 2, pp. 1058–1070. doi: 10.1093/nar/gks1073.
- Reverse gyrase transiently unwinds double-stranded DNA in an ATP-dependent reaction . In: J. Mol. Biol. 425 (), №. - 1, pp. 32–40. doi: .
- DEAD-box helicase conformational changes monitored by single molecule fluorescence resonance energy transfer”, in “RNA Helicases: Analysis of Molecular Mechanisms and Biological Functions . In: Methods in Enzymology 511 (), pp. 75–109. doi: .
- RNA helicases in infection and disease”, Special Issue on “RNA and Disease . In: RNA Biology 9 (), №. - 6, pp. 751–771. doi: .
- The DEAD box helicase eIF4A: paradigm or special case?” for the RNA Biology Special Issue on “RNA helicases . In: RNA Biology 10 (), №. - 1, pp. 19–32. doi: .
- Conformational changes of DEAD-box helicases monitored by single molecule fluorescence resonance energy transfer . In: Methods Enzymol. 511 (), pp. 75–109. doi: .
- The GyrA-box determines the geometry of DNA bound to gyrase and couples DNA binding to the nucleotide cycle . In: Nucleic Acids Res. 40 (), №. - 21, pp. 10893–10903. doi: .
- The DEAD-box helicase elF4A: Paradigm or the odd one out? . In: RNA Biol. 10 (), №. - 1, pp. 1–14. doi: .
- Reverse Gyrase Transiently Unwinds Double-Stranded DNA in an ATP-Dependent Reaction . In: J. Mol. Biol. 425 (), №. - 1, pp. 32–40. doi: .
- Crystal structures of Thermotoga maritima reverse gyrase: inferences for the mechanism of positive DNA supercoiling . In: Nucleic Acids Res. 2012 (), pp. 1–13. doi: .
- Potassium ions are required for nucleotide-induced closure of gyrase N-gate . In: J. Biol. Chem 287 (), №. - 14, pp. 10916–10921. doi: .
- Highlight: Mechanisms of RNA-mediated regulation . In: Biological Chemistry 392 (), №. - 4, p. 275. doi: 10.1515/BC.2011.047.
- Regulatory RNAs and beyond . In: EMBO Reports 12 (), №. - 8, pp. 751–753. doi: 10.1038/embor.2011.150.
- Single-molecule FRET reveals nucleotide-driven conformational changes in molecular machines and their link to RNA unwinding and DNA supercoiling . In: Biochemical Society Transactions 39 (), №. - 2, pp. 611–616. doi: 10.1042/BST0390611.
- Nucleotide-driven conformational changes in the reverse gyrase helicase-like domain couple the nucleotide cycle to DNA processing . In: Physical Chemistry Chemical Physics (), p. Paper. doi: 10.1039/C0CP02859B.
- DNA-induced narrowing of the gyrase N-gate coordinates T-segment capture and strand passage. . In: Proceedings of the National Academy of Sciences of the United States of America 108 (), №. - 34, pp. 14085–14090. doi: 10.1073/pnas.1102100108.
- Guiding strand passage: DNA-induced movement of the gyrase C-terminal domains defines an early step in the supercoiling cycle. . In: Nucleic Acids Research (), pp. 1–14. doi: 10.1093/nar/gkr680.
- The conformational flexibility of the helicase-like domain from Thermotoga maritima reverse gyrase is restricted by the topoisomerase domain . In: Biochemistry 50 (), №. - 26, pp. 5816–5823. doi: .
- eIF4G stimulates the activity of the DEAD box protein eIF4A by a conformational guidance mechanism . In: Nucl. Acids Res. 2011 (), №. - 39(6), pp. 2260–2270. doi: .
- The latch modulates nucleotide and DNA binding to the helicase-like domain of Thermotoga maritima reverse gyrase and is required for positive DNA supercoiling . In: Nucleic Acids Research 39 (), №. - 5, pp. 1789–1800. doi: 10.1093/nar/gkq1048.
- Changing nucleotide specificity of the DEAD-box helicase Hera abrogates communication between the Q-motif and the P-loop . In: Biological Chemistry 329 (), №. - 4, pp. 357–369. doi: 10.1515/BC.2011.034.
- A Structural Model for the DEAD Box Helicase YxiN in Solution: Localization of the RNA Binding Domain . In: Journal of Molecular Biology 402 (), №. - 4, pp. 629–637. doi: 10.1016/j.jmb.2010.07.049.
- The mechanism of ATP-dependent RNA unwinding by DEAD-box proteins . In: Biological Chemistry 390 (), №. - 12, pp. 1237–1250. doi: 10.1515/BC.2009.135.
- (Eds.): Wohin mit uns? – Wissenschaftlerinnen und Wissenschaftler der Zukunft. Lausanne: Peter Lang. , . ISBN 978-3-631-58114-8. doi: .
- The Thermus thermophilus DEAD box helicase Hera contains a modified RNA recognition motif domain loosely connected to the helicase core . In: RNA 15 (), №. - 11, pp. 1993–2001. doi: 10.1261/rna.1820009.
- A conformational change in the helicase core is necessary but not sufficient for RNA unwinding by the DEAD box helicase YxiN . In: Nucleic Acids Research 37 (), №. - 13, pp. 4464–4471. doi: 10.1093/nar/gkp397.
- Crystallization and preliminary characterization of the Thermus thermophilus RNA helicase Hera C-terminal domain . In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications 65 (), №. - 3, pp. 248–252. doi: 10.1107/S1744309108043145.
- A novel dimerization motif in the C-terminal domain of the Thermus thermophilus DEAD box helicase Hera confers substantial flexibility . In: Nucleic Acids Research 37 (), №. - 2, pp. 421–430. doi: 10.1093/nar/gkn947.
- The DNA-gate of Bacillus subtilis gyrase is predominantly in the closed conformation during the DNA supercoiling reaction . In: Proceedings of the National Academy of Sciences of the United States of America 106 (), №. - 32, pp. 13278–13283. doi: 10.1073/pnas.0902493106.
- The DEAD Box Helicase YxiN Maintains a Closed Conformation during ATP Hydrolysis . In: Biochemistry 48 (), №. - 45, pp. 10679–10681. doi: 10.1021/bi901278p.
- The putative RNase P motif in the DEAD box helicase Hera is dispensable for efficient interaction with RNA and helicase activity . In: Nucleic Acids Research 36 (), №. - 18, pp. 5800–5811. doi: 10.1093/nar/gkn581.
- The reverse gyrase helicase-like domain is a nucleotide-dependent switch that is attenuated by the topoisomerase domain . In: Nucleic Acids Research 36 (), №. - 18, pp. 5882–5895. doi: 10.1093/nar/gkn587.
- RNA and ATP cooperatively induce a conformational change in a DEAD box helicase . In: Proceedings of the National Academy of Sciences of the United States of America 105 (), №. - 2, pp. 548–553. doi: 10.1073/pnas.0705488105.
- Adenosine 5′-O-(3-thio)triphosphate (ATPγS) Promotes Positive Supercoiling of DNA by T. maritima Reverse Gyrase . In: Journal of Molecular Biology 371 (), №. - 1, pp. 197–209. doi: 10.1016/j.jmb.2007.05.031.
- Dissection of the Nucleotide Cycle of B. subtilis DNA Gyrase and its Modulation by DNA . In: Journal of Molecular Biology 367 (), №. - 5, pp. 1392–1404. doi: 10.1016/j.jmb.2007.01.055.
- Authentic interdomain communication in an RNA helicase reconstituted by expressed protein ligation of two helicase domains . In: The FEBS Journal 274 (), №. - 2, pp. 463–473. doi: 10.1111/j.1742-4658.2006.05593.x.
- Crystal Structure and Nucleotide Binding of the Thermus thermophilus RNA Helicase Hera N-terminal Domain . In: Journal of Molecular Biology 361 (), №. - 4, pp. 731–743. doi: .
- The tertiary structure of the hairpin ribozyme is formed through a slow conformational search . In: Biochemistry 44 (), №. - 12, pp. 4870–4876. doi: 10.1021/bi047772i.
- A three-fluorophore FRET assay for high-throughput screening of small-molecule inhibitors of ribosome assembly . In: Nucleic Acids Research 32 (), №. - 9, pp. 2707–2715. doi: 10.1093/nar/gkh588.
- DafA cycles between the DnaK chaperone system and translational machinery . In: Journal of Molecular Biology 339 (), №. - 5, pp. 1179–1189. doi: 10.1016/j.jmb.2004.04.052.
- Energetics of hydrogen bond networks in RNA: Hydrogen bonds surrounding G+1 and U42 are the major determinants for the tertiary structure stability of the hairpin ribozyme . In: Biochemistry 41 (), №. - 48, pp. 14095–14102. doi: 10.1021/bi025551b.
- Time-resolved fluorescence resonance energy transfer: A versatile tool for the analysis of nucleic acids . In: Biopolymers 61 (), №. - 3, pp. 159–179. doi: 10.1002/bip.10146.
- Investigation of adenosine base ionization in the hairpin ribozyme by nucleotide analog interference mapping . In: RNA 7 (), №. - 10, pp. 1454–1463. doi: .
- RNA conformation and folding studied with fluorescence resonance energy transfer . In: Methods 23 (), №. - 3, pp. 240–254. doi: 10.1006/meth.2000.1135.
- Tertiary structure stability of the hairpin ribozyme in its natural and minimal forms: Different energetic contributions from a ribose zipper motif . In: Biochemistry 40 (), №. - 37, pp. 11211–11218. doi: 10.1021/bi010773f.
- Tuning of chaperone activity of Hsp70 proteins by modulation of nucleotide exchange . In: Nature Structural Biology 8 (), №. - 5, pp. 427–432. doi: 10.1038/87588.
- Regulation of ATPase and chaperone cycle of DnaK from Thermus thermophilus by the nucleotide exchange factor GrpE . In: Journal of Molecular Biology 305 (), №. - 5, pp. 1173–1183. doi: 10.1006/jmbi.2000.4373.
- Helical junctions as determinants for RNA folding: Origin of tertiary structure stability of the hairpin ribozyme . In: Biochemistry 39 (), №. - 42, pp. 12970–12978. doi: 10.1021/bi0014103.
- Mechanism of regulation of Hsp70 chaperones by DnaJ cochaperones . In: Proceedings of the National Academy of Sciences of the United States of America 96 (), №. - 10, pp. 5452–5457. doi: 10.1073/pnas.96.10.5452.
- The functional cycle and regulation of the Thermus thermophilus DnaK chaperone system . In: Journal of Molecular Biology 287 (), №. - 3, pp. 511–525. doi: 10.1006/jmbi.1999.2636.
- Functional properties of the molecular chaperone DnaK from Thermus thermophilus . In: Journal of Molecular Biology 279 (), №. - 4, pp. 841–853. doi: 10.1006/jmbi.1998.1816.
- Spectroscopic investigations of HIV-1 trans-activator and related peptides in aqueous solutions . In: Biophysical Chemistry 63 (), №. - 2-3, pp. 87–96. doi: 10.1016/S0301-4622(96)02243-0.