2024
Tophoff L, Schüttrumpf H, Heimbecher F, Vollmer C, Philipp B, Abdullaeva Y (2024)
Field investigations and service life assessment of geosynthetic filters in tidally influenced revetments in cases of ochre clogging.
Water 16, 1840. doi.org/10.3390/w16131840Holert J, Borker A, Nübel LL, Daniel R, Poehlein A, Philipp B (2024)
Bacteria use a catabolic patchwork pathway of apparently recent origin for degradation of the synthetic buffer compound TRIS
The ISME Journal, wrad023, https://doi.org/10.1093/ismejo/wrad0232023
Bakenhus I, Jongsma R, Michler-Kozma D, HölscherL, Gabel F, Holert J , Philipp B (2023)
A domesticated photoautotrophic microbial community as a biofilm model system for analyzing the influence of plastic surfaces on invertebrate grazers in limnic environments.
Front. Microbiol. 14:1238913. doi: 10.3389/fmicb.2023.1238913Edelkamp J, Lousada MB, Pinto D, Chéret J, Calabrese FM, Jiménez F, Erdmann H, Wessel J, Philipp B, Angelis M, Rinaldi F, Bertolini M, Paus R (2023)
Management of the human hair follicle microbiome by a synthetic odorant.
J Dermatol Sci 17:S0923-1811(23)00221-9. doi: 10.1016/j.jdermsci.2023.09.006Theodoropoulos E, Walleck W, Kirschke S, Philipp B (2023)
KompoBioPlast@Home? Abbau von Bioplastik im Heimkompost.
BIOspektrum 5/2023, p.549Walleck W, Hasenkamp V, Fuchs D, Philipp B (2023)
Biodialoge zur Bioökonomie und Nachhaltigkeit.
Ökologisches Wirtschaften 2.2023 (38): 30-342022
Weckerle T, Ewald H, Guth P, Knorr KH, Philipp P, Holert J (2022)
Biogas digestate as a sustainable phytosterol source for biotechnological cascade valorization.
Microb Biotechnol 00: 1–13, doi/10.1111/1751-7915.14174Sugden S, Holert J, Cardenas E, Mohn WW, Stein LY (2022)
Microbiome of the freshwater sponge Ephydatia muelleri shares compositional and functional similarities with those of marine sponges.
ISME J 16: 2503–2512, doi: 10.1038/s41396-022-01296-7Czieborowski M, Kempermann AJB, Rolevink E, Blom J, Visser T, Philipp B (2022)
A two-step bioluminescence assay for optimizing antibacterial coating of hollow-fiber membranes with polydopamine in an integrative approach.
J Microbiol Methods 196: 106452, doi: 10.1016/j.mimet.2022.1064522021
Kreutzbruck M, Resch J, Kabasci S, Ivleva NP, Philipp B, Jongsma R, Maga D (2021)
Sachstandspapier zur Bioabbaubarkeit von Kunststoffen.
https://bmbf-plastik.de/de/publikation/qst7-sachstandspapierFeller FM, Eilebracht S, Nedielkov R, Yücel O, Alvincz J, Salinas G, Ludwig KC, Möller H, Philipp B (2021)
Investigations on the Degradation of the Bile Salt Cholate via the 9,10-Seco-Pathway Reveals the Formation of a Novel Recalcitrant Steroid Compund by a Side Reaction in Sphingobium sp. Strain Chol11.
Microorganisms 2021, 9(10), 2146, https://doi.org/10.3390/microorganisms9102146Feller FM, Richtsmeier P, Wege M, Philipp B (2021)
Comparative analysis of bile-salt degradation in Sphingobium sp. strain Chol11 and P. stutzeri Chol1 reveals functional diversity of proteobacterial steroid degradation enzymes and suggests a novel pathway for side-chain degradation.
Appl Environ Microbiol. 2021 Sep 1;AEM0145321.doi: 10.1128/AEM.01453-21Feller FM, Holert J, Yücel O, Philipp B (2021)
Degradation of bile acids by soil and water bacteria.
Microorganisms 2021, 9(8), 1759; https://doi.org/10.3390/microorganisms9081759Feller FM, Wöhlbrand L, Holert J, Schnaars V, Elsner L, Mohn WW, Rabus R, Philipp B (2021)
Proteome, bioinformatic and functional analyses reveal a distinct and conserved metabolic pathway for bile salt degradation in the Sphingomonadaceae.
Appl. Environ. Microbiol. doi: 10.1128/AEM.00987-21Panasia G, Drees SL, Fetzner S, Philipp B (2021)
A complex of LaoA and LaoB acts as a Tat-dependent dehydrogenase for long-chain alcohols in Pseudomonas aeruginosa.
Appl. Environ. Microbiol. doi: 10.1128/AEM.00762-21Bertling J, Bannick CG, Brinkmann L, Barkmann L, Braun U, Knoblauch D, Kraas C, Mederake L, Nosić F, Philipp B, Sartorius I, Schritt H, Stein U, Wencki K, Wendt-Potthoff K, Woidasky J (2021)
Kunststoff in der Umwelt – ein Kompendium, 1. Auflage 2021
doi: 10.24406/umsicht-n-624998Feller FM, Marke G, Drees SL, Wöhlbrand L, Rabus R, Philipp B (2021)
Substrate inhibition of 5β-Δ4-3-ketosteroid dehydrogenase in Sphingobium sp. strain Chol11 acts as circuit breaker during growth with toxic bile salts.
Fron. Microbiol. doi: 10.3389/fmicb.2021.655312Vortmann M, Stumpf AK, Sgobba E, Dirks-Hofmeister ME, Krehenbrink M, Wendisch VF, Philipp B, Moerschbacher BM (2021)
A bottom-up approach towards a bacterial consortium for the biotechnological conversion of chitin to L-lysine.
Applied Microbiology and Biotechnology, doi: 10.1007/s00253-021-11112-5Motealleh A, Dorri P, Czieborowski M, Philipp B, Kehr NS (2021)
Bifunctional nanomaterials for simultaneously improving cell adhesion and affecting bacterial biofilm formation on silicon-based surfaces.
Biomedical Materials, doi: 10.1088/1748-605X/abd872Leiser R, Jongsma R, Bakenhus I, Möckel R, Philipp B, Neu TR, Wendt-Potthoff K (2021)
Interaction of cyanobacteria with calcium facilitates the sedimentation of microplastics in a eutrophic reservoir.
Water Res. 189:116582, doi: 10.1016/j.watres.2020.1165822020
Zecher K, Hayes KR, Philipp B (2020)
Evidence of interdomain ammonium cross-feeding from methylamine- and glycine betaine-degrading Rhodobacteraceae to diatoms as a widespread interaction in the marine phycosphere.
Front. Microbiol. 11: 533894, doi: 10.3389/fmicb.2020.533894Darley PI, Hellstern J, Schink B, Philipp B (2020)
Resorcinol hydroxylase of Azoarcus anaerobius: Molybdenum dependence, activity, and heterologous expression.
Curr. Microbiol., doi: 10.1007/s00284-020-02186-xQin X, Vila-Sanjurjo C, Singh R, Philipp B, Goycoolea FM (2020)
Screening of bacterial quorum sensing inhibitors in a Vibrio fischeri LuxR-based synthetic fluorescent E. coli biosensor
Pharmaceuticals 2020, 13, 263. doi: 10.3390/ph13090263Czieborowski M, Hübenthal A, Poehlein A, Vogt I, Philpp B (2020)
Genetic and physiological analysis of biofilm formation on different plastic surfaces by Sphingomonas sp. strain S2M10 reveals and essential function of sphingan biosynthesis.
Microbiology, doi: 10.1099/mic.0.000961Motealleh A, Czieborowski M, Philipp B, Nowak S, Kehr NS (2020)
Bifunctional Nanomaterials for Enhanced Cell Proliferation and for the Reduction of Bacterial Bioluminescence/Fitness.
Advanced Materials Interfaces 2020, 2000086. doi: 10.1002/admi.2020000862019
Philipp B, Jongsma R (2019)
Untersuchungen zum Mikroplastik in den Rieselfeldern.
Jahresbericht 2018 Biologische Station Rieselfelder Münster, Band 21, S. 100-106Panasia G, Oetermann S, Steinbüchel A, Philipp B (2019)
Sulfate ester detergent degradation in Pseudomonas aeruginosa is subject to both positive and negative regulation.
Applied and Environmental Microbiology 85(23): e01352-19. doi:10.1128/AEM.01352-19Mendelski MN, Dölling R, Feller FM, Hoffmann D, Ramos Fangmeier L, Ludwig KC, Yücel O, Mährlein A, Paul RJ, Philipp B (2019)
Steroids originating from bacterial bile acid degradation affect Caenorhabditis elegans and indicate poteinial risks for the fauna of manured soils.
Scientific Reports 9:11120. doi 10.1038/s41598-019-47476-yStumpf A-K, Vortmann M, Dirks-Hofmeister ME, Moerschbacher B, Philipp B (2019)
Identification of a novel chitinase from Aeromonas hydrophila AH-1N for the degradation of chitin within fungal mycelium.
FEMS Microbiology Letters 366, fny294. doi: 10.1093/femsle/fny294Yücel O, Borgert SR, Poehlein A, Niermann K, Philipp B (2019)
The 7α-hydroxysteroid dehydratase Hsh2 is essential for anaerobic degradation of the steroid skeleton of 7α-hydroxyl bile salts in the novel denitrifying bacterium Azoarcus sp. strain Aa7.
Environmental Microbiology 21: 800-813. doi: 10.1111/1462-2920.145082018
Jagmann N, Philipp B (2018)
SpoT-Mediated Regulation and Amino Acid Prototrophy Are Essential for Pyocyanin Production During Parasitic Growth of Pseudomonas aeruginosa in a Co-culture Model System With Aeromonas hydrophila.
Frontiers in Microbiology 9:761. doi: 10.3389/fmicb.2018.00761Sgobba E, Stumpf A-K, Vortmann M, Jagmann N, Krehenbrink M, Dirks-Hofmeister M, Moerschbacher B, Philipp B and Wendisch VF (2018)
Synthetic Escherichia coli-Corynebacterium glutamicum consortia for L-lysine production from starch and sucrose.
Bioresource Technology 260: 302-310. doi: 10.1016/j.biortech.2018.03.113Panasia G, Philipp B (2018)
Identification of LaoABCR as a novel system for the oxidation of long-chain alcohols derived from SDS- and alkane-degradation in Pseudomonas aeruginosa.
Applied and Environmental Microbiology 84: e00626-18. doi: 10.1128/AEM.00626-18Zecher K, Aitha VP, Heuer K, Ahlers H, Roland K, Fiedel M, Philipp B (2018)
A multi-step approach for testing non-toxic amphiphilic antifouling coatings against marine microfouling at different levels of biological complexity.
Journal of Microbiological Methods 146: 104-114. doi: 10.1016/j.mimet.2018.02.009Warnke M, Jung T, Jacoby C, Agne M, Feller FM, Philipp B, Seiche W, Breit B, Boll M (2018)
Functional characterization of three specific acyl-coenzyme A synthetases involved in anaerobic cholesterol degradation in Sterolibacterium denitrificans Chol1S.
Applied and Environmental Microbiology 84(7): e02721-17. doi: 10.1128/AEM.02721-17Yücel O, Wibberg D, Philipp B, Kalinowski J (2018)
Genome sequence of the bile salt-degrading bacterium Novosphingobium sp. strain Chol11, a model organism for bacterial steroid catabolism.
Genome Announcements 6: e01372-17. doi: 10.1128/genomeA.01372-172017
Yücel O, Holert J, Ludwig KC, Thierbach S, Philipp B (2017)
A novel steroid-coenzyme A ligase from Novosphingobium sp. strain Chol11 is essential for an alternative degradation pathway for bile salts.
Applied and Environmental Microbiology 84(1): e01492-17. doi: 10.1128/AEM.01492-17Zecher K, Suleiman M, Wibberg D, Winkler A, Philipp B, Kalinowski J (2017)
Draft Genome Sequence of Donghicola sp. Strain KarMa, a Model Organism for Monomethylamine-Degrading Nonmethylotrophic Bacteria.
Genome Announcements 5: e01623-16, doi: 10.1128/genomeA.01623-162016
*Suleiman M, *Zecher K, Yücel O, Jagmann N, Philipp B (2016)
Interkindom Cross-Feeding of Ammonium from Marine Methylamine-Degrading Bacteria to the Diatom Phaeodactylum tricornutum.
Applied and Environmental Microbiology 82: 7113-7122. doi:10.1128/AEM.01642-16.
*These authors contributed equally to the paper.Yücel O, Drees S, Jagmann N, Patschkowski T, Philipp B (2016)
An unexplored pathway for degradation of cholate requires a 7α-hydroxysteroid dehydratase and contributes to a broad metabolic repertoire for the utilization of bile salts in Novosphingobium sp. strain Chol11.
Environmental Microbiology 18: 5187-5203. doi: 10.1111/1462-2920.13534Jagmann N, Bleicher V, Busche T, Kalinowski J, Philipp B (2016)
The guanidinobutyrase GbuA is essential for the alkylquinolone-regulated pyocyanin production during parasitic growth of Pseudomonas aeruginosa in co-culture with Aeromonas hydrophila.
Enivronmental Microbiology 18: 3550-3564. doi: 10.1111/1462-2920.13419Holert J, Yücel O, Jagmann N, Prestel A, Möller H, Philipp B (2016)
Identification of bypass reactions leading to the formation of one central steroid degradation intermediate in metabolism of different bile salts in Pseudomonas sp. strain Chol1.
Enivronmental Microbiology 18: 3373-3389. doi: 10.1111/1462-2920.131922015
Zecher K, Jagmann N, Seemann P, Philipp B (2015)
An efficient screening method for the isolation of heterotrophic bacteria influencing growth of diatoms under photoautotrophic conditions.
Journal of Microbiological Methods 119: 154-162. doi: 10.1016/j.mimet.2015.10.016Molina-Fuentes Á, Pacheco D, Marín P, Philipp B, Schink B, Marqués S (2015)
Identification of the gene cluster for the anaerobic degradation of 3,5-dihydroxybenzoate (α-resorcylate) in Thauera aromatica strain AR-1.
Applied and Environmental Microbiology 81: 7201-7214. doi: 10.1128/AEM.01698-15Jagmann N, Henke SF, Philipp B (2015)
Cells of Escherichia coli are protected against severe chemical stress by co-habiting cell aggregates formed by Pseudomonas aeruginosa.
Applied Microbiology and Biotechnology 99: 8285-8294 doi: 10.1007/s00253-015-6726-72014
Windler M, Leinweber K, Rio Bartulos C, Philipp B, Kroth PG (2014)
Biofilm and capsule formation of the diatom Achnanthidium minutissimum are effected by a bacterium.
Journal of Phycology 51: 343-355. doi: 10.1111/jpy.12280Jagmann N, Philipp B (2014)
Design of synthetic microbial communities for biotechnological production processes.
Journal of Biotechnology 184: 209-218. doi: 10.1016/j.biotec.2014.05.019*Holert J, *Yücel O, Suvekbala V, Kulic Z, Möller HM, Philipp B (2014)
Evidence of distinct pathways for bacterial degradation of the steroid compound cholate indicates the potential for metabolic interactions by interspecies cross-feeding.
Environmental Microbiology 16: 1424-1440. doi: 10.1111/1462-2920.12407
*These authors contributed equally to the paper.Barrientos A, Merino, E,Casabon I, Rodriguez J, Crowe AM, Holert J, Philipp B, Eltis LD, Olivera ER, Luengo JM (2014)
Functional analysis of three acyl-CoA synthetases involved in bile acids degradation in Pseudomonas putida DOC21.
Environmental Microbiology 17: 47-63. doi: 10.1111/1462-2920.123952013
Hauck D, Joachim I, Frommeyer B, Varrot A, Philipp B, Möller HM, Imberty A, Exner TE, Titz A (2013)
Discovery of two classes of potent glycomimetic inhibitors of Pseudomonas aeruginosa LecB with distinct binding modes.
ACS Chemical Biology 8: 1775-1784. doi: 10.1021/cb400371rHolert J, Jagmann N, Philipp B (2013)
The essential function of genes for a hydratase and an aldehyde dehydrogenase for growth of Pseudomonas sp. strain Chol1 with the steroid compound cholate indicates an aldolytic reaction step for deacetylation of the side chain.
Journal of Bacteriology 195: 3371-3380. doi: 10.1128/JB.00410-13Jagmann N, Philipp B (2013)
Fightin' for chitin: Wie Bakterien beim Abbau von Polymeren konkurrieren.
BIOspektrum 2/13: 137-139. doi: 10.1007/s12268-013-0282-4Holert J, Kulic Z, Yücel O, Suvekbala V, Suter MJ-F, Möller H, Philipp B (2013)
Degradation of the acyl side chain of the steroid compound cholate in Pseudomonas sp. strain Chol1 proceeds via an aldehyde intermediate.
Journal of Bacteriology 195: 585-595. doi: 10.1128/JB.01961-12Holert J, Alam I, Larsen M, Antunés A, Bajic VB, Stingl U, Philipp B (2013)
Genome sequence of Pseudomonas sp. strain Chol1, a model organism for the degradation of bile salts and other steroid compounds.
Genome Announcements 1: e00014-12. doi: 10.1128/genomeA.00014-122012
Philipp B, Schink B (2012)
Different strategies in anaerobic biodegradation of aromatic compounds: nitrate reducers versus strict anaerobes.
Environmental Microbiology Reports, 4: 469-478. doi: 10.1111/j.1758-2229.2011.00304.xJagmann N, von Rekowski KS, Philipp B (2012)
Interactions of bacteria with different mechanisms for chitin degradation result in the formation of a mixed-species biofilm.
FEMS Microbiology Letters 326: 69-7. doi: 10.1111/j.1574-6968.2011.02435.x1998-2011
Birkenmaier A, Müller H, Philipp B (2011)
Identification of a thiolase gene essential for beta-oxidation of the acyl side chain of the steroid compound cholate in Pseudomonas sp. strain Chol1.
FEMS Microbiology Letters 318: 123-130. doi: 10.1111/j.1574-6968.2011.02250.xPhilipp B (2011)
Bacterial degradation of bile salts.
Applied Microbiology and Biotechnology 89: 903-915. doi: 10.1007/s00253-010-2998-0Jagmann N, Brachvogel H-P, B Philipp (2010)
Parasitic growth of Pseudomonas aeruginosa in co-culture with the chitinolytic bacterium Aeromonas hydrophila.
Environmental Microbiology 12: 1787-1802. doi: 10.1111/j.1462-2920.2010.02271.xKlebensberger J, Birkenmaier A, Geffers R, Kjelleberg S, Philipp B (2009)
SiaA and SiaD are essential for inducing autoaggregation as a specific response to detergent stress in Pseudomonas aeruginosa.
Environmental Microbiology 11: 3073-3086. doi: doi: 10.1111/j.1462-2920.2009.02012.xPhilipp B (2009)
Zellaggregation als Überlebensstrategie beim Wachstum mit Tensiden.
BIOspektrum 6/2009: 696Styp von Rekowski K, Hempel M, Philipp B (2008)
Quorum sensing by N-acylhomoserine lactones is not required for Aeromonas hydrophila during growth with organic particles in lake water microcosms.
Archives of Microbiology 189: 475-482. doi: 10.1007/s00203-007-0338-2Birkenmaier A, Holert J, Erdbrink H, Möller H, Friemel A, Schönenberger R, Suter MJ-F, Klebensberger J, Philipp B (2007)
Biochemical and genetic investigation on initial reactions in aerobic degradation of the bile acid cholate in Pseudomonas sp. strain Chol1.
Journal of Bacteriology 189: 7165-7173. doi: 10.1128/JB.00665-07Darley PI, Hellstern JA, Medina-Bellver JI, Marqués S, Schink B, Philipp B (2007)
Heterologous expression and identification of the genes involved in anaerobic degradation of 1,3-dihydroxybenzene (Resorcinol) in Azoarcus anaerobius.
Journal of Bacteriology 189: 3824-3833. doi: 10.1128/JB.01729-06Klebensberger J, Lautenschlager K, Bressler D, Wingender J and Philipp B (2007)
Detergent-induced cell aggregation in subpopulations of Pseudomonas aeruginosa as a pre-adaptive survival strategy.
Environmental Microbiology 9: 2247-2259. doi: 10.1111/j.1462-2920.2007.01339.xMüller N, Hempel M, Philipp B, Gross E (2007)
Degradation of gallic acid and hydolysable polyphenols is constitutively activated in the freshwater plant-associated bacterium Matsuebacter sp. FB25.
Aquatic Microbial Ecology 47: 83-97. doi: 10.3354/ame047083Philipp B, Hoff M, Germa F, Schink B, Beimborn D, Mersch-Sundermann V (2007)
Biochemical interpretation of quantitative structure activity relationships (QSAR) for biodegradation of N-heterocycles: A complementary approach to predict biodegradability.
Environmental Science & Technology 41: 1390-1398. doi: 10.1021/es061505dKlebensberger J, Rui O, Fritz E, Schink B, Philipp B (2006)
Cell aggregation of Pseudomonas aeruginosa strain PAO1 as an energy-dependent stress response during growth with sodium dodecyl sulfate.
Archives of Microbiology 185: 417-427. doi: 10.1007/s00203-006-0111-yPhilipp B, Erdbrink H, Suter MJF, Schink B (2006)
Degradation of and sensitivity to cholate in Pseudomonas sp. strain Chol1.
Archives of Microbiology 185: 192-201. doi: 10.1007/s00203-006-0085-9Chhabra SR, Philipp B, Eberl L, Givskov M, Williams P, Cámara M (2005)
Extracelluar communication in bacteria. In: Schulz S (ed.) The chemistry of pheromones and other semiochemicals II.
Topics in Current Chemistry 240: 279-315. doi: 10.1007/b98319Yates EA, Philipp B, Buckley C, Atkinson S, Chhabra SR, Sockett RE, Goldner M , Dessaux Y , Cámara M, Smith H, Williams P (2002)
N-Acylhomoserine lactones undergo lactonolysis in a pH, temperature and acyl chain length dependent manner during growth of Yersinia pseudotuberculosis and Pseudomonas aeruginosa.
Infection and Immunity 70: 5635-46. doi: 10.1128/iai.70.10.5635-5646.2002Philipp B, Kemmler D, Hellstern J, Gorny N, Caballero A, Schink B (2002)
Anaerobic degradation of protocatechuate (3,4-dihydroxybenzoate) by Thauera aromatica strain AR-1.
FEMS Microbiology Letters 212: 139-143. doi: 10.1111/j.1574-6968.2002.tb11257.xRorije E , Germa F, Philipp B, Schink B, Beimborn DB (2002)
Predicting biodegradability from structure: Imidazoles.
SAR & QSAR in Environmental Research 13: 199-204. doi: 10.1080/10629360290002271Bussmann I , Philipp B, Schink B (2001)
Factors influencing the cultivability of lake water bacteria.
Journal of Microbiological Methods 47: 41-50. doi: 10.1016/s0167-7012(01)00289-5Reichenbecher W, Philipp B, Suter M, Schink B (2000)
Hydroxyhydroquinone reductase, the first enzyme in hydroxyhydroquinone (1,2,4-trihydroxy-benzene) degradation by Desulfovibrio inopinatus.
Archives of Microbiology 173: 206-212. doi: 10.1007/s002039900130Esteve-Nunez A , Lucchesi G, Philipp B, Schink B, Ramos JL (2000)
Respiration of 2,4,6-trinitrotoluene by Pseudomonas sp. strain JLR11.
Journal of Bacteriology 182: 1352-1355. doi: 10.1128/jb.182.5.1352-1355.2000Schink B, Philipp B, Müller J (2000)
Anaerobic degradation of phenolic compounds.
Naturwissenschaften 87: 12-23. doi: 10.1007/s001140050002Philipp B, Schink B (2000)
Two distinct pathways for anaerobic degradation of aromatic compounds by the denitrifying bacterium Thauera aromatica strain AR-1.
Archives of Microbiology 173: 91-96. doi: 10.1007/s002039900112Springer N, Ludwig W, Philipp B, Schink B (1998)
Azoarcus anaerobius sp. nov.: A resorcinol-degrading, strictly anaerobic denitrifying bacterium.
International Jounal of Systematic and Evolutionary Microbiology 48: 953-956. doi: 10.1099/00207713-48-3-953Philipp B, Schink B (1998)
Evidence of two oxidative reactions initiating anaerobic degradation of resorcinol (1,3 dihydroxybenzene) by the denitrifying bacterium Azoarcus anaerobius.
Journal of Bacteriology 180: 3644-3649. doi: 10.1128/JB.180.14.3644-3649.1998Wehrmann A, Philipp B, Sahm H, Eggeling L (1998)
The different modes of diaminopimelate (m-DAP) synthesis and their role in cell wall integrity: A study with Corynebacterium glutamicum.
Journal of Bacteriology 180: 3159-3165. doi: 10.1128/JB.180.12.3159-3165.1998