Prof. Dr. Christos Gatsogiannis

Prof. Dr. Christos Gatsogiannis

Busso-Peus-Straße 10, room 110.001
48149 Münster

T: +49 251 83-34411

Academic Profile

External Profile

  • Research Areas

    • Structural Biology
    • Biochemistry
    • cryoEM
    • Peroxisomes
    • AAA ATPases
    • Membrane proteins
    • Protein import

  • CV

    Education

    PhD (Dr. rer. nat., summa cum laude), Johannes Gutenberg University Mainz, Germany
    Study of Biology (Dipl.), Johannes-Gutenberg-University Mainz, Germany

    Positions

    Co-Director of the Center for Soft Nanoscience
    Member: CiM Graduate School and CiMIC Interfaculty Cente CiM: Cluster of excellence „Cells in Motion”
    Professor (W3) at the University of Münster
    Project Group Leader, Department of Structural Biochemistry, Max Planck Institute of Molecular Physiology, Dortmund, Germany
    Postdoc, Max Planck Institute of Molecular Physiology, Dortmund, Germany

  • Teaching

    • Praktikum: *+) Cryo-electron microscopy: Structural studies of macromolecular machines at atomic resolution [134390]
      [n. V. | Prof. Dr. Christos Gatsogiannis]
    • Praktikum: *+) Cryo-electron microscopy: Structural studies of macromolecular machines at atomic resolution [132308]
      [n. V. | Prof. Dr. Christos Gatsogiannis]
    • Praktikum: *+) Cryo-electron microscopy: Structural studies of macromolecular machines at atomic resolution [130449]
    • Praktikum: *+) Cryo-electron microscopy: Structural studies of macromolecular machines at atomic resolution [138375]
    • Praktikum: *+) Cryo-electron microscopy: Structural studies of macromolecular machines at atomic resolution [136328]

  • Publications

    • Bourque C; Klink BU; Gatsogiannis C. . ‘An AI-assisted cryo-EM pipeline for structural studies of cellular extracts.’ Structure 30, No. 4: 532–534. doi: 10.1016/j.str.2022.03.016.
    • Günther P; Quentin D; Ahmad S; Sachar K; Gatsogiannis C; Whitney JC; Raunser S. . ‘Structure of a bacterial Rhs effector exported by the type VI secretion system.’ PLoS Pathogens 18, No. 1: e1010182. doi: 10.1371/journal.ppat.1010182.
    • Klink BU; Herrmann E; Antoni C; Langemeyer L; Kiontke S; Gatsogiannis C; Ungermann C; Raunser S; Kümmel D. . ‘Structure of the Mon1-Ccz1 complex reveals molecular basis of membrane binding for Rab7 activation.’ Proceedings of the National Academy of Sciences of the United States of America 119, No. 6. doi: 10.1073/pnas.2121494119.
    • Rüttermann M; Gatsogiannis C. . ‘Good things come to those who bait: the peroxisomal docking complex.’ Biological Chemistry 404(2-3): 107–119. doi: 10.1515/hsz-2022-0161.
    • Skoneczny M; Francisco T; Erdmann R; Gatsogiannis C. . ‘Editorial: Structural aspects of peroxisome biogenesis and functions.’ Frontiers in cell and developmental biology 10: 1114759. doi: 10.3389/fcell.2022.1114759.
    • Wagner M; Blum D; Raschka SL; Nentwig L; Gertzen CGW; Chen M; Gatsogiannis C; Harris A; Smits SHJ; Wagner R; Schmitt L. . ‘A New Twist in ABC Transporter Mediated Multidrug Resistance - Pdr5 is a Drug/proton Co-transporter.’ Journal of Molecular Biology 434, No. 14: 167669. doi: 10.1016/j.jmb.2022.167669.

    • Lill P, Hansen T, Wendscheck D, Klink BU, Jeziorek T, Vismpas D, Miehling J, Bender J, Schummer A, Drepper F, Girzalsky W, Warscheid B, Erdmann R, Gatsogiannis C. . ‘Towards the molecular architecture of the peroxisomal receptor docking complex.’ Proceedings of the National Academy of Sciences of the United States of America 2020. doi: 10.1073/pnas.2009502117.
    • Klink BU, Gatsogiannis C, Hofnagel O, Wittinghofer A, Raunser S. . ‘Structure of the human BBSome core complex.’ eLife 9. doi: 10.7554/eLife.53910.
    • Stabrin M, Schoenfeld F, Wagner T, Pospich S, Gatsogiannis C, Raunser S. . ‘TranSPHIRE: automated and feedback-optimized on-the-fly processing for cryo-EM.’ Nature Communications 11, No. 1: 5716. doi: 10.1038/s41467-020-19513-2.
    • Antoni C, Quentin D, Lang AE, Aktories K, Gatsogiannis C, Raunser S. . ‘Cryo-EM structure of the fully-loaded asymmetric anthrax lethal toxin in its heptameric pre-pore state.’ PLoS Pathogens 16, No. 8: e1008530. doi: 10.1371/journal.ppat.1008530.

    • Wagner T, Merino F, Stabrin M, Moriya T, Antoni C, Apelbaum A, Hagel P, Sitsel O, Raisch T, Prumbaum D, Quentin D, Roderer D, Tacke S, Siebolds B, Schubert E, Shaikh TR, Lill P, Gatsogiannis C, Raunser S. . ‘SPHIRE-crYOLO is a fast and accurate fully automated particle picker for cryo-EM.’ Communications biology 2: 218. doi: 10.1038/s42003-019-0437-z.
    • Gatsogiannis C, Balogh D, Merino F, Sieber SA, Raunser S. . ‘Cryo-EM structure of the ClpXP protein degradation machinery.’ Nature structural {&} molecular biology 26, No. 10: 946–954. doi: 10.1038/s41594-019-0304-0.
    • Leidreiter F, Roderer D, Meusch D, Gatsogiannis C, Benz R, Raunser S. . ‘Common architecture of Tc toxins from human and insect pathogenic bacteria.’ Science advances 5, No. 10: eaax6497. doi: 10.1126/sciadv.aax6497.
    • Tanaka Y, Kato S, Stabrin M, Raunser S, Matsui T, Gatsogiannis C. . ‘Cryo-EM reveals the asymmetric assembly of squid hemocyanin.’ IUCrJ 6, No. Pt 3: 426–437. doi: 10.1107/S205225251900321X.

    • Vinayagam D, Mager T, Apelbaum A, Bothe A, Merino F, Hofnagel O, Gatsogiannis C, Raunser S. . ‘Electron cryo-microscopy structure of the canonical TRPC4 ion channel.’ eLife 7. doi: 10.7554/eLife.36615.
    • Gatsogiannis C, Merino F, Roderer D, Balchin D, Schubert E, Kuhlee A, Hayer-Hartl M, Raunser S. . ‘Tc toxin activation requires unfolding and refolding of a \textgreekb-propeller.’ Nature 563, No. 7730: 209–213. doi: 10.1038/s41586-018-0556-6.
    • Kato S, Matsui T, Gatsogiannis C, Tanaka Y. . ‘Molluscan hemocyanin: structure, evolution, and physiology.’ Biophysical Reviews 10, No. 2: 191–202. doi: 10.1007/s12551-017-0349-4.
    • Pfeifer W, Lill P, Gatsogiannis C, Sacc{à} B. . ‘Hierarchical Assembly of DNA Filaments with Designer Elastic Properties.’ ACS Nano 12, No. 1: 44–55. doi: 10.1021/acsnano.7b06012.
    • Saha S, Holzapfel B, Chen Y, Terlinden K, Lill P, Gatsogiannis C, Rehage H, Clever GH. . ‘Rational Design of an Amphiphilic Coordination Cage-Based Emulsifier.’ Journal of the American Chemical Society 140, No. 50: 17384–17388. doi: 10.1021/jacs.8b10991.

    • Chen M, Kato K, Kubo Y, Tanaka Y, Liu Y, Long F, Whitman WB, Lill P, Gatsogiannis C, Raunser S, Shimizu N, Shinoda A, Nakamura A, Tanaka I, Yao M. . ‘Structural basis for tRNA-dependent cysteine biosynthesis.’ Nature Communications 8, No. 1: 1521. doi: 10.1038/s41467-017-01543-y.
    • Efremov RG, Gatsogiannis C, Raunser S. . ‘Lipid Nanodiscs as a Tool for High-Resolution Structure Determination of Membrane Proteins by Single-Particle Cryo-EM.’ Methods in Enzymology 594: 1–30. doi: 10.1016/bs.mie.2017.05.007.
    • Moriya T, Saur M, Stabrin M, Merino F, Voicu H, Huang Z, Penczek PA, Raunser S, Gatsogiannis C. . ‘High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE.’ Journal of visualized experiments : JoVE 2017, No. 123. doi: 10.3791/55448.
    • Sprengel A, Lill P, Stegemann P, Bravo-Rodriguez K, Schönewei{ß} E, Merdanovic M, Gudnason D, Aznauryan M, Gamrad L, Barcikowski S, Sanchez-Garcia E, Birkedal V, Gatsogiannis C, Ehrmann M, Sacc{à} B. . ‘Tailored protein encapsulation into a DNA host using geometrically organized supramolecular interactions.’ Nature Communications 8: 14472. doi: 10.1038/ncomms14472.

    • Gatsogiannis C, Merino F, Prumbaum D, Roderer D, Leidreiter F, Meusch D, Raunser S. . ‘Membrane insertion of a Tc toxin in near-atomic detail.’ Nature structural {&} molecular biology 23, No. 10: 884–890. doi: 10.1038/nsmb.3281.

    • Gatsogiannis C, Hofnagel O, Markl J, Raunser S. . ‘Structure of mega-hemocyanin reveals protein origami in snails.’ Structure (London, England : 1993) 23, No. 1: 93–103. doi: 10.1016/j.str.2014.10.013.
    • Poepsel S, Sprengel A, Sacca B, Kaschani F, Kaiser M, Gatsogiannis C, Raunser S, Clausen T, Ehrmann M. . ‘Determinants of amyloid fibril degradation by the PDZ protease HTRA1.’ Nature Chemical Biology 11, No. 11: 862–869. doi: 10.1038/nchembio.1931.
    • Raunser S, Gatsogiannis C. . ‘Deciphering the tubulin code.’ Cell 161, No. 5: 960–961. doi: 10.1016/j.cell.2015.05.004.

    • Erkelenz M, Bauer DM, Meyer R, Gatsogiannis C, Raunser S, Sacc{à} B, Niemeyer CM. . ‘A facile method for preparation of tailored scaffolds for DNA-origami.’ Small (Weinheim an der Bergstrasse, Germany) 10, No. 1: 73–77. doi: 10.1002/smll.201300701.
    • Meusch D, Gatsogiannis C, Efremov RG, Lang AE, Hofnagel O, Vetter IR, Aktories K, Raunser S. . ‘Mechanism of Tc toxin action revealed in molecular detail.’ Nature 508, No. 7494: 61–65. doi: 10.1038/nature13015.

    • Gatsogiannis C, Lang AE, Meusch D, Pfaumann V, Hofnagel O, Benz R, Aktories K, Raunser S. . ‘A syringe-like injection mechanism in Photorhabdus luminescens toxins.’ Nature 495, No. 7442: 520–523. doi: 10.1038/nature11987.
    • Sadian Y, Gatsogiannis C, Patasi C, Hofnagel O, Goody RS, Farkasovský M, Raunser S. . ‘The role of Cdc42 and Gic1 in the regulation of septin filament formation and dissociation.’ eLife 2: e01085. doi: 10.7554/eLife.01085.

    • Bröcker C, Kuhlee A, Gatsogiannis C, Balderhaar HJk, Hönscher C, Engelbrecht-Vandré S, Ungermann C, Raunser S. . ‘Molecular architecture of the multisubunit homotypic fusion and vacuole protein sorting (HOPS) tethering complex.’ Proceedings of the National Academy of Sciences of the United States of America 109, No. 6: 1991–1996. doi: 10.1073/pnas.1117797109.

    • Lieb B, Gebauer W, Gatsogiannis C, Depoix F, Hellmann N, Harasewych MG, Strong EE, Markl J. . ‘Molluscan mega-hemocyanin: an ancient oxygen carrier tuned by a {\~{}}}550 kDa polypeptide.’ Frontiers in Zoology 7: 14. doi: 10.1186/1742-9994-7-14.

    • Gatsogiannis C, Markl J. . ‘Keyhole limpet hemocyanin: 9-A CryoEM structure and molecular model of the KLH1 didecamer reveal the interfaces and intricate topology of the 160 functional units.’ Journal of Molecular Biology 385, No. 3: 963–983. doi: 10.1016/j.jmb.2008.10.080.

    • Gatsogiannis C, Moeller A, Depoix F, Meissner U, Markl J. . ‘Nautilus pompilius hemocyanin: 9 A cryo-EM structure and molecular model reveal the subunit pathway and the interfaces between the 70 functional units.’ Journal of Molecular Biology 374, No. 2: 465–486. doi: 10.1016/j.jmb.2007.09.036.
    • Meissner U, Gatsogiannis C, Moeller A, Depoix F, Harris JR, Markl J. . ‘Comparative 11A structure of two molluscan hemocyanins from 3D cryo-electron microscopy.’ Micron 38, No. 7: 754–765. doi: 10.1016/j.micron.2006.11.005.