Name: Susanne Bomke
Diploma / M.Sc degree: Westfälische Wilhelms-Universität Münster, Germany
(February 2007)

PhD Project: Metallocenes as labels in bioanalysis


Homepage: http://www.uni-muenster.de/Chemie.ac/karst/group/bomke.html

Abstract of Research Project

Nowadays, metallocene derivatives have shown a growing, not yet fully investigated, potential as labels in bioanalysis. Metallocene derivatives based on ferrocenes and charged cobaltocinium cations containing succinimidester or maleimide groups are used for the derivatization of amines and thiols. These compounds show a high stability over the complete analytical process and are soluble in polar organic solvents and even water. Thus, excellent labeling conditions for functional groups in biomolecules, e.g. proteins, are provided, rendering possible the identification and quantification of these groups in complex biosamples.

On this background, my work contains the synthesis of several derivatizat ion reagents and the development of different analytical methods.

Therefore, a method for the determination of biogenic amines in food samples, which is based on a calibration by stable isotope-labeled internal standards should be developed. Furthermore, my task is to develop a new strategy for the analysis of peptides and proteins using metallocene derivatives as selective labeling reagents. The labeled analytes can be characterized with liquid chromatography (LC) and capillary electrophoresis (CE) with electrospray (ESI) and inductively coupled plasma (ICP) mass spectrometry (MS) detection. Thereby, the use of LC/MS provides the molecular structure, whereas the ICP-MS measurements complete the analysis by giving the exact quantitative information of the metal content in the sample.


Publications

S. Bomke, B. Seiwert, L. Dudek, S. Effkemann, U. Karst
Liquid chromatography/mass spectrometry method for the determination of biogenic amines in food samples
Anal. Bioanal. Chem. 393 (2009), 247-256.


Susanne Bomke
eMail: Susanne Bomke