Publikationen
2023
Sadhanasatish T, Augustin K, Windgasse L, Chrostek-Grashoff A, Rief M, Grashoff C. A molecular optomechanics approach reveals functional relevance of force transduction across talin and desmoplakin. Sci Adv. 2023 Jun 23;9(25):eadg3347. [Epub 2023 Jun 21]
Windgasse L, Grashoff C. Multiplexed Molecular Tension Sensor Measurements Using PIE-FLIM. Methods Mol Biol. 2023;2600:221-237. [Epub 2023 Jan 1]
2022
Bauer N, Maisuls I, Pereira da Graça A, Reinhardt D, Erapaneedi R, Kirschnick N, Schäfers M, Grashoff C, Landfester K, Vestweber D, Strassert CA, Kiefer F. Genetically encoded dual fluorophore reporters for graded oxygen-sensing in light microscopy. Biosens Bioelectron. 2023 Feb 1;221:1149170. [Epub 2022 Nov 17]
2021
Glogowska E, Arhatte M, Chatelain FC, Lesage F, Xu A, Grashoff C, Discher DE, Patel A, Honoré E. Piezo1 and Piezo2 foster mechanical gating of K2P channels Tissues. Cell Rep. 2021 Nov 30.;37(9):110070.
Fischer LS, Schlichthaerle T, Chrostek-Grashoff A, Grashoff C. Peptide-PAINT Enables Investigation of Endogenous Talin with Molecular Scale Resolution in Cells and Tissues. Chembiochem. 2021 Oct 1;22(19):2872:2879. [Epub 2021 Jul 30]
Fischer LS, Rangarajan S, Sadhanasatish T, Grashoff C. Molecular Force Measurement with Tension Sensors. Annu Rev Biophys. 2021 May 6;50:595-616. [Epub 2021 Mar 12]
Arif N, Zinnhardt M, Nyamay'Antu A, Teber D, Brückner R, Schaefer K, Li YT, Trappmann B, Grashoff C, Vestweber D. PECAM-1 supports leukocyte diapedesis by tension-dependent dephosphorylation of VE-cadherin. EMBO J. 2021 May 3;40(9):e106113. [Epub 2021 Feb 19]
Fischer LS, Klingner C, Strauss MT, Böttcher R, Fässler R, Jungmann R, Grashoff C. Quantitative single-protein imaging reveals molecular complex formation of integrin, talin, and kindlin during cell adhesion. Nat Commun. 2021 Feb 10;12(1): 919.
2020
Kanoldt V, Kluger C, Barz C, Schweizer AL, Ramanujam D, Windgasse L, Engelhardt S, Chrostek-Grashoff A, Grashoff C. Metavinculin modulates force transduction in cell adhesion sites. Nat Commun. 2020 Dec 17;11(1): 6403.
2019
Lemke SB, Weidemann T, Cost AL, Grashoff C, Schnorrer F. A small proportion of Talin molecules transmit forces at developing muscle attachments in vivo. PLoS Biol. 2019 Mar 27;17(3):e3000057.
Cost AL, Khalaji S, Grashoff C. Genetically Encoded FRET-Based Tension Sensors. Curr Protoc Cell Biol. 2019 Jun;83(1):e85. [Epub 2019 Mar 13]
2018
Price AJ, Cost AL, Ungewiß H, Waschke J, Dunn AR, Grashoff C. Mechanical loading of desmosomes depends on the magnitude and orientation of external stress. Nat Commun. 2018 Dec 11;9(1):5284.
Kanoldt V, Fischer L, Grashoff C. Unforgettable force - crosstalk and memory of mechanosensitive structures. Biol Chem. 2019 May 27;400(6):687-698. [Epub 2018 Nov 1]
2017
Ringer P, Weißl A, Cost AL, Freikamp A, Sabass B, Mehlich A, Tramier M, Rief M, Grashoff C. Multiplexing molecular tension sensors reveals piconewton force gradient across talin-1. Nature Methods 2017 Nov; 14(11):1090-1096. [Epub 2017 Sep 18] »
Ringer P, Colo G, Fässler R, Grashoff C. Sensing the mechano-chemical properties of the extracellular matrix. Matrix Biol. 2017 Dec; 64:6-16. [Epub 2017 Apr 4] »
Freikamp A, Mehlich A, Klingner C, Grashoff C. Investigating piconewton forces in cells by FRET-based molecular force microscopy. J. Struct. Biol. 2017 Jan; 197(1):37-42. [Epub 2016 Mar 12] »
2016
Freikamp A, Cost AL, Grashoff C. The Piconewton Force Awakens: Quantifying Mechanics in Cells. Trends Cell Biol. 2016 Nov; 26(11):838-847. [Epub 2016 Aug 17] »
2015
Austen K, Ringer P, Mehlich A, Chrostek-Grashoff A, Kluger C, Klingner C, Sabass B, Zent R, Rief M, Grashoff C. Extracellular rigidity sensing by talin isoform-specific mechanical linkages. Nat Cell Biol. 2015 Dec; 17(12):1597-1606. [Epub 2015 Nov 2] »
Cost AL, Ringer P, Chrostek-Grashoff A, Grashoff C. How to Measure Molecular Forces in Cells: A Guide to Evaluating Genetically-Encoded FRET-Based Tension Sensors. Cell Mol Bioeng. 2015; 8(1):96-105. [Epub 2014 Dec 2] »
2014
Leerberg JM, Gomez GA, Verma S, Moussa EJ, Wu SK, Priya R, Hoffman BD, Grashoff C, Schwartz MA, Yap AS. Tension-sensitive actin assembly supports contractility at the epithelial zonula adherens. Curr Biol. 2014 Aug 4; 24(15):1689-1699. [Epub 2014 Jul 24] »
2013
Kayser J, Haslbeck M, Dempfle L, Krause M, Grashoff C, Buchner J, Herrmann H, Bausch AR. The small heat shock protein Hsp27 affects assembly dynamics and structure of keratin intermediate filament networks. Biophys J. 2013 Oct 15; 105(8):1778-1785. »
Austen K, Kluger C, Freikamp A, Chrostek-Grashoff A, Grashoff C. Generation and Analysis of Biosensors to Measure Mechanical Forces Within Cells. Methods Mol Biol. 2013; 1066:169-184. »
Elad N, Volberg T, Patla I, Hirschfeld-Warneken V, Grashoff C, Spatz JP, Fässler R, Geiger B, Medalia O. The role of integrin-linked kinase in the molecular architecture of focal adhesions. J Cell Sci. 2013 Sep 15; 126(Pt 18):4099-4107. [Epub 2013 Jul 10] »
Moik D, Böttcher A, Makhina T, Grashoff C, Bulus N, Zent R, Fässler R. Mutations in the Paxillin-binding Site of Integrin-linked Kinase (ILK) Destabilize the Pseudokinase Domain and Cause Embryonic Lethality in Mice. J Biol Chem. 2013 Jun 28; 288(26):18863-18871. [Epub 2013 May 8] »
2012
Azimifar SB, Böttcher RT, Zanivan S, Grashoff C, Krüger M, Legate KR, Mann M, Fässler R. Induction of membrane circular dorsal ruffles requires co-signalling of integrin-ILK-complex and EGF receptor. J Cell Sci. 2012 Jan 15; 125(Pt 2):435-448. »
2011
Hoffman BD, Grashoff C, Schwartz MA. Dynamic molecular processes mediate cellular mechanotransduction. Nature. 2011 Jul 20; 475:316-323. »
2010
Patla I, Volberg T, Elad N, Hirschfeld-Warneken V, Grashoff C, Fassler R, Spatz JP, Geiger B, Medalia O. Dissecting the molecular architecture of integrin adhesion sites by cryo-electron tomography. Nat Cell Biol. 2010 Sep; 12(9):909-915. [Epub 2010 Aug 8] »
Grashoff C, Hoffman BD, Brenner MD, Zhou R, Parsons M, Yang MT, McLean MA, Sligar SG, Chen CS, Ha T, Schwartz MA. Measuring mechanical tension across vinculin reveals regulation of focal adhesion dynamics. Nature. 2010 Jul 8; 466:263-266. »
Hortelano S, Lopez-Fontal R, Traves PG, Villa N, Grashoff C, Bosca L, Luque A. ILK mediates LPS-induced vascular adhesion receptor expression and subsequent leukocyte trans-endothelial migration. Cardiovasc Res. 2010 May 1; 86:283-292. [Epub 2010 Feb 17] »
2009
Stanchi F, Grashoff C, Nguemeni Yonga CF, Grall D, Fassler R, Van Obberghen-Schilling E. Molecular dissection of the ILK-PINCH-parvin triad reveals a fundamental role the ILK kinase domain in the late stages of focal-adhesion maturation. J Cell Sci. 2009 Jun 1; 122:1800-1811. [Epub 2009 May 12] »
Bergmann S, Lang A, Rohde M, Agarwal V, Rennemeier C, Grashoff C, Preissner KT, Hammerschmidt S. Integrin-linked kinase is required for vitronectin-mediated internalization of Streptococcus pneumoniae by host cells. J Cell Sci. 2009 Jan 15; 122:256-267. »
2008
Maier S, Lutz R, Gelman L, Sarasa-Renedo A, Schenk S, Grashoff C, Chiquet M. Tenascin-C induction by cyclic strain requires integrin-linked kinase. Biochim Biophys Acta. 2008 Jun; 1783:1150-1162. [Epub 2008 Jan 26] »
2007
Lorenz K, Grashoff C, Torka R, Sakai T, Langbein L, Bloch W, Aumailley M, Fassler R. Integrin-linked kinase is required for epidermal and hair follicle morphogenesis. J Cell Biol. 2007 May 7; 177:501-513. »
2005
Gimona M, Grashoff C, Kopp P. Oktoberfest for adhesion structures. EMBO Rep. 2005 Oct; 6:922-926. »
2004
Grashoff C, Thievessen I, Lorenz K, Ussar S, Fassler R. Integrin-linked kinase: integrin's mysterious partner. Curr Opin Cell Biol. 2004 Oct; 16:565-571. »
2003
Grashoff C, Aszodi A, Sakai T, Hunziker EB, Fassler R. Integrin-linked kinase regulates chondrocyte shape and proliferation. EMBO Rep. 2003 Apr; 4:432-438. »
Sakai T, Li S, Docheva D, Grashoff C, Sakai K, Kostka G, Braun A, Pfeifer A, Yurchenco PD, Fassler R. Integrin-linked kinase (ILK) is required for polarizing the epiblast, cell adhesion, and controlling actin accumulation. Genes Dev. 2003 Apr 1; 17:926-940. »